Sinop University Faculty of Fisheries, Department of Fish Diseases, Sinop, Turkey
Malatya Turgut Özal University, Vahap Küçük Vocational High School, Malatya, Turkey
Turkiye Parazitol Derg. 2023 Mar 2;47(1):42-48. doi: 10.4274/tpd.galenos.2022.30301.
The aim of this study is to investigate in detail the morphological features of the digenean parasite isolated from the European anchovy using light and scanning electron microscopy (SEM).
The specimens of were obtained from the pharynx and stomach of the European caught by commercial fishing vessels in the Black Sea. Parasites were killed in a hot normal saline solution, preserved in to 70% ethanol for light microscopic (LM) studies, and in 2.5% glutaraldehyde for SEM. The morphological diagnostic features of were studied in detail under both LM and SEM.
The morphological characteristics of the examined adult specimens were found to be similar to the original descriptions describing the basic characteristics of forebody and hindbody shape, the position and shape of the vitellarium, ovary, and and testes, and the shapes of oral and ventral sucker. The measurement data for all morphological diagnostics were provided; photomicrographs of each part of the parasite were presented. Infection prevalence, mean intensity and mean abundance values were 8.89%, 4.5 and 0.4, respectively.
All available records of morphology are based on light microscope, this is the first study to identification the morphological features of the parasite with SEM. This research is the first on presence in on the Turkish Black Sea coast.
本研究旨在通过光镜和扫描电子显微镜（SEM）详细研究从欧洲鳀鱼中分离出的双腔吸虫寄生虫的形态特征。
从在黑海被商业渔船捕获的欧洲鳀鱼的咽部和胃中获得的标本。寄生虫在热生理盐水溶液中死亡，保存在 70%乙醇中用于光镜（LM）研究，保存在 2.5%戊二醛中用于 SEM。在 LM 和 SEM 下详细研究了的形态诊断特征。
检查的成年寄生虫标本的形态特征与描述前体和后体形状、卵黄腺、卵巢和睾丸以及口吸盘和腹吸盘位置和形状的原始描述相似。提供了所有形态诊断的测量数据；呈现了寄生虫各部分的显微照片。感染率、平均强度和平均丰度值分别为 8.89%、4.5 和 0.4。
所有现有的关于形态的记录都基于光学显微镜，这是首次使用 SEM 鉴定寄生虫形态特征的研究。这是关于土耳其黑海沿岸存在的首次研究。
