一种基于硫代磷酸酯-DNA和硫结合结构域的快速核酸检测平台。
A rapid nucleic acid detection platform based on phosphorothioate-DNA and sulfur binding domain.
作者信息
Shuai Yuting, Ju Yi, Li Yuanhang, Ma Dini, Jiang Lan, Zhang Jingyu, Tan Gao-Yi, Liu Xueting, Wang Shenlin, Zhang Lixin, Liu Guang
机构信息
State Key Laboratory of Bioreactor Engineering, and School of Biotechnology, East China University of Science and Technology (ECUST), Shanghai, 200237, China.
State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic &Developmental Sciences, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
出版信息
Synth Syst Biotechnol. 2023 Feb 11;8(2):213-219. doi: 10.1016/j.synbio.2023.02.002. eCollection 2023 Jun.
Nucleic acid detection plays a key role in diverse diagnosis and disease control. Currently available nucleic acid detection techniques are challenged by trade-offs among speed, simplicity, precision and cost. Here, we described a novel method, designated SENSOR (Sulfur DNA mediated nucleic acid sensing platform), for rapid nucleic acid detection. SENSOR was developed from phosphorothioate (PT)-DNA and sulfur binding domain (SBD) which specifically binds double-stranded PT-modified DNA. SENSOR utilizes PT-DNA oligo and SBD as targeting module, which is linked with split luciferase reporter to generate luminescence signal within 10 min. We tested detection on synthesized nucleic acid and COVID-19 pseudovirus, achieving attomolar sensitivity combined with an amplification procedure. Single nucleotide polymorphisms (SNP) could also be discriminated. Indicating SENSOR a new promising nucleic acid detection technique.
核酸检测在各种诊断和疾病控制中起着关键作用。目前可用的核酸检测技术面临着速度、简便性、精度和成本之间权衡的挑战。在此，我们描述了一种名为SENSOR（硫代DNA介导的核酸传感平台）的新型快速核酸检测方法。SENSOR由硫代磷酸酯（PT）-DNA和特异性结合双链PT修饰DNA的硫结合结构域（SBD）开发而来。SENSOR利用PT-DNA寡核苷酸和SBD作为靶向模块，与分裂荧光素酶报告基因相连，在10分钟内产生发光信号。我们对合成核酸和新冠病毒假病毒进行了检测，结合扩增程序实现了阿托摩尔灵敏度。单核苷酸多态性（SNP）也能够被区分。这表明SENSOR是一种有前景的新型核酸检测技术。
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