2009年甲型H1N1流感大流行病毒在中国的遗传多样性、复制及传播情况
The genetic diversity, replication, and transmission of 2009 pandemic H1N1 viruses in China.
作者信息
Sun Hailiang, Wang Yongcui, Liu Hanlin, Pang Zifeng, Cui Xinxin, Zhao Rui, Liu Yanwei, Qu Xiaoyun, Huang Mian, Ke Changwen, Liao Ming
机构信息
College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, South China Agricultural University, Guangzhou, China.
出版信息
Front Microbiol. 2023 Feb 17;14:1110100. doi: 10.3389/fmicb.2023.1110100. eCollection 2023.
BACKGROUND
The 2009 pandemic H1N1 influenza A virus (pdm09) continue to evolve, and few studies have systemically analyzed the evolution, replication, and transmission of pmd09 viruses in China.
METHODS
To better understand the evolution and pathogenicity of pdm09 viruses, we systematically analyzed viruses that were confirmed in 2009-2020 in China and characterized their replication and transmission ability. We extensively analyzed the evolution characteristics of pdm/09 in China over the past decades. The replication ability of 6B.1 and 6B.2 lineages on Madin-Darby canine kidney (MDCK) and human lung adenocarcinoma epithelial (A549) cells and their pathogenicity and transmission in guinea pigs were also compared.
RESULTS
In total, 3,038 pdm09 viruses belonged to clade 6B.1 (62% of all pdm09 viruses) and clade 6B.2 (4%). Clade 6B.1 pdm09 viruses are the predominant clade, with proportions of 54.1%, 78.9%, 57.2%, 58.6%, 61.7%, 76.3%, and 66.6% in the North, Northeast, East, Central, South, Southwest, and Northeast regions in China, respectively. The isolation proportion of clade 6B.1 pdm/09 viruses was 57.1%, 74.3%, 96.1%, 98.2%, 86.7%, and 78.5% in 2015-2020, respectively. A clear differentiation time point appeared in 2015 before which the evolution trend of pdm09 viruses in China was similar to that in North America but then showed a different trend after that point. To characterize pdm09 viruses in China after 2015, we further analyzed 33 pdm09 viruses isolated in Guangdong in 2016-2017, among which A/ Guangdong/33/2016 and A/Guangdong/184/2016 (184/2016) belonged to clade 6B.2, and the other 31 strains belonged to clade 6B.1. A/Guangdong/887/2017 (887/2017) and A/Guangdong/752/2017 (752/2017) (clade 6B.1), 184/2016 (clade 6B.2) and A/California/04/2009 (CA04) replicated efficiently in MDCK cells and A549 cells, as well as the turbinates of guinea pigs. 184/2016 and CA04 could transmit among guinea pigs through physical contact.
CONCLUSION
Our findings provide novel insights into the evolution, pathogenicity, and transmission of pdm09 virus. The results show that enhancing surveillance of pdm09 viruses and timely evaluation of their virulence are essential.
背景
2009年甲型H1N1流感大流行病毒（pdm09）持续演变，而很少有研究系统分析pdm09病毒在中国的进化、复制和传播情况。
方法
为了更好地了解pdm09病毒的进化和致病性，我们系统分析了2009年至2020年在中国确诊的病毒，并对其复制和传播能力进行了表征。我们广泛分析了过去几十年中国pdm/09的进化特征。还比较了6B.1和6B.2谱系在犬肾传代细胞（MDCK）和人肺腺癌上皮细胞（A549）上的复制能力及其在豚鼠中的致病性和传播情况。
结果
总共3038株pdm09病毒属于6B.1分支（占所有pdm09病毒的62%）和6B.2分支（4%）。6B.1分支的pdm09病毒是主要分支，在中国北方、东北、东部、中部、南部、西南和西北地区分别占54.1%、78.9%、57.2%、58.6%、61.7%、76.3%和66.6%。2015 - 2020年期间，6B.1分支pdm/09病毒的分离比例分别为57.1%、74.3%、96.1%、98.2%、86.7%和78.5%。2015年出现了一个明显的分化时间点，在此之前中国pdm09病毒的进化趋势与北美相似，但之后呈现出不同趋势。为了表征2015年后中国的pdm09病毒，我们进一步分析了2016 - 2017年在广东分离的33株pdm09病毒，其中A/广东/33/2016和A/广东/184/2016（184/2016）属于6B.2分支，其他31株属于6B.1分支。A/广东/887/2017（887/2017）和A/广东/752/2017（752/2017）（6B.1分支）、184/2016（6B.2分支）和A/加利福尼亚/04/2009（CA04）在MDCK细胞、A549细胞以及豚鼠鼻甲中均能高效复制。184/2016和CA04可通过身体接触在豚鼠之间传播。
结论
我们的研究结果为pdm09病毒的进化、致病性和传播提供了新的见解。结果表明，加强对pdm09病毒的监测并及时评估其毒力至关重要。
Zotero 插件