Bratisl Lek Listy. 2023;124(6):437-441. doi: 10.4149/BLL_2023_066.
Fibronectin (Fn) is a glycoprotein of extracellular matrix produced by a variety of mesenchymal and neoplastic cell types.
In adult brain tissue, Fn is restricted to blood vessels. However, adult human brain cultures are almost entirely comprised of flat or spindle‑shaped Fn-positive cells usually referred to as "glia-like" cells. Since Fn is primarily present in fibroblasts, these cultures may be considered to be of non-glial origin.
Cells gained by long-term culturing of adult human brain tissue derived from brain biopsies obtained from 12 patients with non-malignant diagnoses were examined by immunofluorescence methods.
Primary cultures contained GFAP-/Vim+/Fn+ "glia-like" cells (95-98 %) and GFAP+/Vim+/Fn- astrocytes (0.1 %) which disappeared by passage number 3. The formation of cell processes and enlargement of cell bodies was observed in 9 of 12 cultures with decreased cell growth during passages 12 to 17. It is remarkable that during this period, all "glia-like" cells became GFAP+/Vim+/Fn+.
Herein, we confirm our previously published hypothesis about the origin of adult human "glia-like" cells, which we consider to be precursor cells scattered through the brain cortex and subcortical white matter. Cultures were comprised entirely of GFAP-/Fn+ "glia-like" cells and showed morphological and immunochemical astroglial differentiation with spontaneously decelerated growth during prolonged passaging. We propose that the adult human brain tissue contains a "dormant population" of undefined glial precursor cells. Under culture, these cells show to have a high proliferative capacity and different stages of cell dedifferentiation (Fig. 2, Ref. 21).
纤连蛋白（Fn）是一种细胞外基质糖蛋白，由多种间充质和肿瘤细胞类型产生。
在成人脑组织中，Fn 局限于血管。然而，成人脑组织培养物几乎完全由扁平或梭形 Fn 阳性细胞组成，通常称为“类胶质细胞”。由于 Fn 主要存在于成纤维细胞中，这些培养物可被认为是非神经胶质来源的。
通过对从 12 名非恶性诊断的脑活检获得的成人脑组织进行长期培养获得的细胞，通过免疫荧光方法进行检查。
原代培养物含有 GFAP-/Vim+/Fn+“类胶质”细胞（95-98%）和 GFAP+/Vim+/Fn-星形胶质细胞（0.1%），这些细胞在传代 3 时消失。在 12 个培养物中的 9 个中观察到细胞突起的形成和细胞体的增大，在传代 12 到 17 期间细胞生长减少。值得注意的是，在此期间，所有“类胶质”细胞均成为 GFAP+/Vim+/Fn+。
在此，我们证实了我们之前关于成人“类胶质”细胞起源的假设，我们认为它们是散在分布于大脑皮质和皮质下白质中的前体细胞。培养物完全由 GFAP-/Fn+“类胶质”细胞组成，表现出形态和免疫化学星形胶质分化，在长时间传代过程中生长自然减慢。我们提出，成人脑组织中存在一种未定义的胶质前体细胞“休眠群体”。在培养条件下，这些细胞具有高增殖能力和不同阶段的细胞去分化（图 2，参考文献 21）。
