Wei Xiaojing, Yu Leilei, Zhang Chuan, Ni Yongqing, Zhao Jianxin, Zhang Hao, Chen Wei, Zhai Qixiao, Tian Fengwei
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
Carbohydr Polym. 2023 Jan 1;299:120156. doi: 10.1016/j.carbpol.2022.120156. Epub 2022 Oct 7.
Chitooligosaccharides (COS) have many bioactive functions and favorable prospects in the fields of biomedicine and functional foods. In this study, COS was found to significantly improve the survival rate of neonatal necrotizing enterocolitis (NEC) model rats, alter the composition of the intestinal microbiota, inhibit the expression of inflammatory cytokines, and alleviate intestinal pathological injury. In addition, COS also increased the abundance of Akkermansia, Bacteroides, and Clostridium sensu stricto 1 in the intestines of normal rats (the normal rat model is more universal). The in vitro fermentation results found that COS was degraded by the human gut microbiota to promote the abundance of Clostridium sensu stricto 1 and produced numerous short-chain fatty acids (SCFAs). In vitro metabolomic analysis revealed that COS catabolism was associated with significant increases in 3-hydroxybutyrate acid and γ-aminobutyric acid. This study provides evidence for the potential of COS as a prebiotic in food products and to ameliorate NEC development in neonatal rats.
壳寡糖（COS）在生物医药和功能性食品领域具有多种生物活性功能和良好前景。在本研究中，发现COS可显著提高新生鼠坏死性小肠结肠炎（NEC）模型大鼠的存活率，改变肠道微生物群的组成，抑制炎性细胞因子的表达，并减轻肠道病理损伤。此外，COS还增加了正常大鼠肠道中阿克曼氏菌、拟杆菌和严格梭菌1的丰度（正常大鼠模型更具普遍性）。体外发酵结果发现，COS被人体肠道微生物群降解，从而促进严格梭菌1的丰度增加，并产生大量短链脂肪酸（SCFAs）。体外代谢组学分析显示，COS分解代谢与3-羟基丁酸和γ-氨基丁酸的显著增加有关。本研究为COS作为食品中益生元以及改善新生大鼠NEC发展的潜力提供了证据。
