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商业水凝胶的系统比较表明，层粘连蛋白和应变硬化的协同作用促进了神经细胞的定向迁移。

Systematic Comparison of Commercial Hydrogels Revealed That a Synergy of Laminin and Strain-Stiffening Promotes Directed Migration of Neural Cells.

机构信息

Research Laboratory of the Department of Plastic, Reconstructive and Aesthetic Surgery, Medical University of Vienna, Vienna 1090, Austria.

Austrian Cluster for Tissue Regeneration, Vienna 1200, Austria.

出版信息

ACS Appl Mater Interfaces. 2023 Mar 15;15(10):12678-12695. doi: 10.1021/acsami.2c20040. Epub 2023 Mar 6.

DOI:10.1021/acsami.2c20040
PMID:36876876
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10020957/
Abstract

Hydrogels have shown potential in replacing damaged nerve tissue, but the ideal hydrogel is yet to be found. In this study, various commercially available hydrogels were compared. Schwann cells, fibroblasts, and dorsal root ganglia neurons were seeded on the hydrogels, and their morphology, viability, proliferation, and migration were examined. Additionally, detailed analyses of the gels' rheological properties and topography were conducted. Our results demonstrate vast differences on cell elongation and directed migration on the hydrogels. Laminin was identified as the driver behind cell elongation and in combination with a porous, fibrous, and strain-stiffening matrix structure responsible for oriented cell motility. This study improves our understanding of cell-matrix interactions and thereby facilitates tailored fabrication of hydrogels in the future.

摘要

水凝胶在替代受损神经组织方面显示出了潜力，但理想的水凝胶仍有待发现。在这项研究中，比较了各种市售的水凝胶。雪旺细胞、成纤维细胞和背根神经节神经元被接种到水凝胶上，检测了它们的形态、活力、增殖和迁移。此外，还对凝胶的流变学性质和形貌进行了详细分析。我们的结果表明，细胞在水凝胶上的伸长和定向迁移存在巨大差异。层粘连蛋白被确定为细胞伸长的驱动因素，与多孔、纤维状和应变硬化基质结构相结合，负责细胞的定向运动。这项研究提高了我们对细胞-基质相互作用的理解，从而有助于未来定制水凝胶的制造。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d83/10020957/9d1159a6218a/am2c20040_0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d83/10020957/866f7385d31e/am2c20040_0002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d83/10020957/26154647293e/am2c20040_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d83/10020957/2bbf4749e240/am2c20040_0008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d83/10020957/9d1159a6218a/am2c20040_0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d83/10020957/866f7385d31e/am2c20040_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d83/10020957/91343afd12a3/am2c20040_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d83/10020957/e301d547de2b/am2c20040_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d83/10020957/0b1151084d5b/am2c20040_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d83/10020957/c43ec6adf970/am2c20040_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d83/10020957/26154647293e/am2c20040_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d83/10020957/2bbf4749e240/am2c20040_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d83/10020957/ca48ba1473f1/am2c20040_0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d83/10020957/9d1159a6218a/am2c20040_0010.jpg

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