Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan.
Chemical Materials Development Department, TANAKA KIKINZOKU KOGYO K.K., Tsukuba Technical Center, 22 Wadai, Tsukuba, Ibaraki 300-4247, Japan.
Chem Commun (Camb). 2023 Mar 30;59(27):4000-4003. doi: 10.1039/d2cc06933d.
Herein, we report two newly synthesized silver cluster-assembled materials (SCAMs), [Ag(SBu)(CFCOO)(bpa)] (bpa = 1,2-bis(4-pyridyl)acetylene) and [Ag(SBu)(CFCOO)(bpeb)] (bpeb = 1,4-bis(pyridin-4-ylethynyl)benzene) composed of Ag and Ag chalcogenolate cluster cores, respectively, bridged by acetylenic bispyridine linkers. The linker structures and electrostatic interaction between positively charged SCAMs and negatively charged DNA confer the SCAMs with the ability to suppress the high background fluorescence of single-stranded (ss) DNA probes with SYBR Green I nucleic acid stain, leading to high signal-to-noise ratio for label-free target DNA detection.
在此，我们报告了两种新合成的银簇组装材料（SCAMs），[Ag(SBu)(CFCOO)(bpa)]（bpa = 1,2-双（4-吡啶基）乙炔）和[Ag(SBu)(CFCOO)(bpeb)]（bpeb = 1,4-双（吡啶-4-基乙炔基）苯），分别由 Ag 和 Ag 硫属簇核组成，由炔基双吡啶连接体桥接。连接体结构和带正电荷的 SCAMs 与带负电荷的 DNA 之间的静电相互作用赋予了 SCAMs 抑制 SYBR Green I 核酸染料单链（ss）DNA 探针高背景荧光的能力，从而为无标记靶 DNA 检测提供了高信噪比。
