Faculty of Biomedical Engineering, Toin University of Yokohama.
J Oleo Sci. 2023;72(3):295-301. doi: 10.5650/jos.ess22260.
We investigated the skin permeation of 5-aminolevulinic acid (ALA) through Yucatan micropig full-thickness skin by using ALA-loaded W/O nanoemulsions composed of Span/Tween/ethanol (EtOH)/isopropyl palmitate (IPP)/10 wt% aqueous ALA solution. The nanoemulsions were prepared using Span 20/Tween 20 (S20/T20), Span 80/Tween 80 (S80/T80), and Span 20/Tween 80 (S20/T80) mixed surfactant systems. Based on the results of the phase diagram study and hydrodynamic diameter measurement of the nanoemulsions, we decided that the optimal weight ratio of Span/Tween/EtOH/IPP/10 wt% aqueous ALA solution in the nanoemulsion was 0.8/0.2/14/19/1.4. The permeability coefficient of ALA in the S20/T80 system was approximately five times larger than those in the S20/T20 and S80/T80 systems. The high skin permeation of ALA afforded by the ALA-loaded W/O nanoemulsion in the S20/T80 system is attributable to a significant enhancement in the partitioning of ALA to the stratum corneum.
我们通过使用由 Span/Tween/乙醇（EtOH）/异丙基棕榈酸酯（IPP）/10wt%水性 ALA 溶液组成的负载 ALA 的 W/O 纳米乳研究了 5-氨基乙酰丙酸（ALA）通过 Yucatan 微型猪全厚皮肤的渗透。纳米乳是使用 Span 20/Tween 20（S20/T20）、Span 80/Tween 80（S80/T80）和 Span 20/Tween 80（S20/T80）混合表面活性剂系统制备的。基于相图研究和纳米乳的水动力直径测量的结果，我们决定纳米乳中 Span/Tween/EtOH/IPP/10wt%水性 ALA 溶液的最佳重量比为 0.8/0.2/14/19/1.4。在 S20/T80 系统中，ALA 的渗透系数大约是 S20/T20 和 S80/T80 系统的五倍。S20/T80 系统中负载 ALA 的 W/O 纳米乳赋予 ALA 的高皮肤渗透性归因于 ALA 向角质层的分配显著增强。
