直接检测人类乙醛脱氢酶-2（ALDH2）基因座上的常见和非典型等位基因。
Direct detection of usual and atypical alleles on the human aldehyde dehydrogenase-2 (ALDH2) locus.
作者信息
Hsu L C, Bendel R E, Yoshida A
机构信息
Beckman Research Institute of the City of Hope, Durate, CA 91010.
出版信息
Am J Hum Genet. 1987 Dec;41(6):996-1001.
A method for determining human mitochondrial aldehyde dehydrogenase (ALDH2) genotypes was developed. Two 21-base synthetic oligonucleotides, one complementary to the usual ALDH2(1) gene and the other complementary to the atypical ALDH2(2) gene, were used as specific probes for in-gel hybridization analysis of human genomic DNA from either peripheral blood cells or livers. Under appropriate hybridization conditions, these two probes can hybridize to their specific complementary alleles and thus allow the genotyping of the ALDH2 locus.
开发了一种确定人类线粒体醛脱氢酶（ALDH2）基因型的方法。使用两条21个碱基的合成寡核苷酸，一条与常见的ALDH2(1)基因互补，另一条与非典型的ALDH2(2)基因互补，作为特异性探针，用于对外周血细胞或肝脏来源的人类基因组DNA进行凝胶内杂交分析。在适当的杂交条件下，这两条探针可与其特定的互补等位基因杂交，从而实现ALDH2基因座的基因分型。
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