Department of Food & Nutrition, Lady Irwin College, New Delhi, India.
Department of Biomedical Science, Dr. Ambedkar Centre for Biomedical Research, North Campus, University of Delhi, New Delhi, India.
Food Sci Technol Int. 2024 Dec;30(8):699-712. doi: 10.1177/10820132231159819. Epub 2023 Mar 6.
Plant-based protein sources as a sustainable alternative to animal sources are highly relevant for food and dietary supplements industries. Plant proteins are becoming popular as an eco-friendly source for meeting global protein requirements due to their importance in nutrition, management of metabolic diseases, biological activities, functionality in processed food products and their low carbon footprints. We applied biochemical protein extraction protocol and prepared protein concentrate from an underutilised cereal, foxtail millet, with plausible applications in foods and supplements. Herein efforts were utilised to obtain foxtail millet protein (FMP) concentrate by means of standardisation of processes of extraction cum isolation. The conditions including flour to solvent ratio, extraction-precipitation pH, dissolution time, etc. were optimised to significantly improve protein yield and recovery. The FMP concentrate prepared was also analysed for nutritional composition, bioactive compounds, amino acid content and digestion properties in comparison to packaged brown rice protein concentrate. The protein concentrate prepared was found to have high digestibility, rich in essential amino acids with good phenolic and flavonoid content, thereby making it a potential sensory and antioxidant additive for food/pharmaceutical applications.
植物性蛋白质来源作为动物来源的可持续替代品,对食品和膳食补充剂行业具有重要意义。由于植物蛋白在营养、代谢性疾病管理、生物活性、加工食品中的功能以及低碳足迹方面的重要性,它们作为一种环保的蛋白质来源,正日益受到欢迎,以满足全球蛋白质需求。我们应用生化蛋白质提取方案,从一种未充分利用的谷物——黍米中制备了蛋白质浓缩物,这种浓缩物在食品和补充剂中有潜在的应用。本文努力通过提取和分离过程的标准化来获得黍米蛋白(FMP)浓缩物。优化了包括面粉与溶剂比、提取-沉淀 pH 值、溶解时间等条件,以显著提高蛋白质的产率和回收率。还分析了 FMP 浓缩物的营养成分、生物活性化合物、氨基酸含量和消化特性,并与包装糙米蛋白浓缩物进行了比较。结果表明,制备的蛋白质浓缩物具有较高的消化率,富含必需氨基酸,具有良好的酚类和类黄酮含量,因此它是一种有潜力的感官和抗氧化添加剂,可用于食品/制药应用。