B细胞免疫球蛋白特征改变在人类结直肠癌中具有潜在诊断价值。

Altered B cell immunoglobulin signature exhibits potential diagnostic values in human colorectal cancer.

作者信息

Liu Rui-Xian, Wen Chuangyu, Ye Weibiao, Li Yewei, Chen Junxiong, Zhang Qian, Li Weiqian, Liang Wanfei, Wei Lili, Zhang Jingdan, Chan Ka-Wo, Wang Xueqin, Yang Xiangling, Liu Huanliang

机构信息

Department of Clinical Laboratory, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China.

Guangdong Institute of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China.

出版信息

iScience. 2023 Feb 3;26(3):106140. doi: 10.1016/j.isci.2023.106140. eCollection 2023 Mar 17.

Antibody-secreting B cells have long been considered the central element of gut homeostasis; however, tumor-associated B cells in human colorectal cancer (CRC) have not been well characterized. Here, we show that the clonotype, phenotype, and immunoglobulin subclasses of tumor-infiltrating B cells have changed compared to adjacent normal tissue B cells. Remarkably, the tumor-associated B cell immunoglobulin signature alteration can also be detected in the plasma of patients with CRC, suggesting that a distinct B cell response was also evoked in CRC. We compared the altered plasma immunoglobulin signature with the existing method of CRC diagnosis. Our diagnostic model exhibits improved sensitivity compared to the traditional biomarkers, CEA and CA19-9. These findings disclose the altered B cell immunoglobulin signature in human CRC and highlight the potential of using the plasma immunoglobulin signature as a non-invasive method for the assessment of CRC.

长期以来，分泌抗体的B细胞一直被认为是肠道内环境稳定的核心要素；然而，人类结直肠癌（CRC）中肿瘤相关B细胞的特征尚未得到充分描述。在此，我们表明，与相邻正常组织B细胞相比，肿瘤浸润B细胞的克隆型、表型和免疫球蛋白亚类发生了变化。值得注意的是，在CRC患者的血浆中也能检测到肿瘤相关B细胞免疫球蛋白特征的改变，这表明CRC中也引发了独特的B细胞反应。我们将改变后的血浆免疫球蛋白特征与现有的CRC诊断方法进行了比较。与传统生物标志物癌胚抗原（CEA）和糖类抗原19-9（CA19-9）相比，我们的诊断模型具有更高的灵敏度。这些发现揭示了人类CRC中B细胞免疫球蛋白特征的改变，并突出了将血浆免疫球蛋白特征用作评估CRC的非侵入性方法的潜力。