评价侧向流动法和 ELISA 技术在检测土耳其 COVID-19 病例 IgG 和 IgM 抗体中的应用。

Evaluation of lateral flow and ELISA techniques for detecting IgG and IgM antibodies in COVID-19 cases in Türkiye.

机构信息

DESAM Research Institute, Near East University, Nicosia, Cyprus.

Department of Biomedical Engineering, Izmir University of Economics, Izmir, Türkiye.

出版信息

East Mediterr Health J. 2023 Feb 26;29(2):91-99. doi: 10.26719/emhj.23.011.

DOI:10.26719/emhj.23.011

PMID:36880490

Abstract

BACKGROUND

Antibody testing can complement molecular assays for detecting COVID-19.

AIMS

We evaluated the concurrence between lateral flow assay and enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies in severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).

METHODS

The study was conducted at Kocaeli University, Türkiye. We used a lateral flow assay and ELISA to test serum samples from COVID-19 cases, confirmed by polymerase chain reaction assays (study group) and pre-pandemic stored serum samples (control group). We used Deming regression to evaluate the antibody measurements.

RESULTS

The study group included 100 COVID-19 cases, and the control group included pre-pandemic samples from 156 individuals. The lateral flow assay detected immunoglobulin M (IgM) and G (IgG) antibodies in 35 and 37 study group samples. ELISA detected IgM nucleocapsid (N) antibodies in 18 samples, and IgG (N) and IgG spike 1 (S1) antibodies in 31 and 29 samples, respectively. None of the techniques detected antibodies in the control samples. Strong correlations were found between lateral flow IgG (N+ receptor-binding domain + S1) and ELISA IgG (S) (r = 0.93, P < 0.01) and ELISA IgG (N) (r = 0.81, P < 0.01). Weaker correlations were seen between ELISA IgG S and IgG N (r = 0.79, P < 0.01) and lateral flow assay and ELISA IgM (N) (r = 0.70, P < 0.01).

CONCLUSION

Lateral flow assay and ELISA techniques gave consistent results for IgG/IgM antibody measurements towards spike and nucleocapsid proteins, suggesting that both methods can be used to detect COVID-19 where access to molecular test kits is difficult.

摘要

背景

抗体检测可补充用于检测 COVID-19 的分子检测。

目的

我们评估侧向流动分析与酶联免疫吸附测定（ELISA）在检测严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）抗体方面的一致性。

方法

该研究在土耳其科贾埃利大学进行。我们使用侧向流动分析和 ELISA 测试了聚合酶链反应检测（研究组）和大流行前储存血清样本（对照组）确诊的 COVID-19 病例的血清样本。我们使用 Deming 回归来评估抗体测量。

结果

研究组包括 100 例 COVID-19 病例，对照组包括大流行前的 156 人样本。侧向流动分析在 35 个和 37 个研究组样本中检测到免疫球蛋白 M（IgM）和 G（IgG）抗体。ELISA 检测到 18 个样本的 IgM 核衣壳（N）抗体，以及 31 个和 29 个样本的 IgG（N）和 IgG 刺突 1（S1）抗体。两种技术均未在对照组样本中检测到抗体。侧向流动 IgG（N+受体结合域+S1）与 ELISA IgG（S）之间存在很强的相关性（r=0.93，P<0.01），与 ELISA IgG（N）之间也存在很强的相关性（r=0.81，P<0.01）。ELISA IgG S 和 IgG N（r=0.79，P<0.01）以及侧向流动分析和 ELISA IgM（N）之间的相关性较弱（r=0.70，P<0.01）。