Al-Manara College for Medical Sciences, Maysan, Iraq.
Medical Laboratory Techniques Department, College of Medical Techology, Al-Farahidi University, Baghdad, Iraq.
Arch Razi Inst. 2022 Aug 31;77(4):1453-1457. doi: 10.22092/ARI.2022.359086.2369. eCollection 2022 Aug.
are characterized as rod- shaped, motile, gram- negative bacteria which has the ability to infect animals and human. occasionally causes sickness while in most cases not lead to severe symptoms. Analyzing milk for spp. is not routine but traditional culture methods are used to evaluate the health condition of the dairy products. However, the antibody-based and nucleic-acid- based methods are practical for identifying spp. Therefore, this research was designed to evaluate the use of traditional culture methods and PCR in detection of the presence of spp. in raw milk samples in, Maysan Iraq. A total number of 130 raw milk samples collected from Maysan Iraq. All the samples were analyzed for the presence of spp. using traditional culture method and polymerase chain reaction (PCR). The culture method used in this experiment were done by using pre-enrichment, enrichment, selective plating and biochemical tests. The results of this traditional technique were compared with the results obtained from PCR method. The PCR was performed using a 284bp sequence of the gene. The results showed that 8 (7.07%) of samples were identified as positive using traditional culture technique but 14 (12.3%) samples were detected as positive by PCR method. The results of the current research revealed that the traditional culture based methods are generally time costuming and labor intensive but the development of new rapid methods including DNA based methods such as PCR are more sensitive and have dramatically decreased the time necessary for the detection of bacteria.
这些细菌呈杆状、可移动、革兰氏阴性，具有感染动物和人类的能力。偶尔会引起疾病，但在大多数情况下不会导致严重症状。分析牛奶中的 spp. 并不常见，但传统的培养方法用于评估乳制品的健康状况。然而，基于抗体和基于核酸的方法更适用于鉴定 spp.。因此，本研究旨在评估传统培养方法和 PCR 在检测伊拉克迈桑省生奶样品中 spp. 的存在。从伊拉克迈桑省采集了 130 份生奶样品。所有样品均采用传统培养方法和聚合酶链反应（PCR）检测 spp. 的存在。本实验中使用的培养方法包括预增菌、增菌、选择性平板和生化试验。将传统技术的结果与 PCR 方法获得的结果进行比较。PCR 采用 284bp 的 基因序列进行。结果表明，8（7.07%）份样品经传统培养技术鉴定为 spp. 阳性，但 14（12.3%）份样品经 PCR 方法检测为 spp. 阳性。本研究结果表明，基于传统培养的方法通常耗时且劳动强度大，但包括 PCR 在内的新快速方法的发展更灵敏，并大大缩短了检测细菌所需的时间。
