Department of Clinical Biochemistry, College of Medicine, University of Thi-Qar, Thi-Qar, Iraq.
Department of Chemistry, College of Sciences, University of Baghdad, Baghdad, Iraq.
Arch Razi Inst. 2022 Aug 31;77(4):1341-1348. doi: 10.22092/ARI.2022.357640.2080. eCollection 2022 Aug.
Breast cancer is the most common malignancy affecting women's health, with an increasing incidence worldwide. This study aimed to measure the intracellular concentration of the hypoxia-inducible factor 1 α (HIF-1α), tumor suppression protein p53, and estradiol (E2) in tumor tissues of adult females with breast cancer and their relation to tumor grade, tumor size, and lymph node metastases (LNM). The study was conducted on 65 adult female participants with breast mass admitted to the operating theater in Al-Hussein Teaching Hospital and Al-Habboby Teaching Hospital in Nasiriyah, Iraq, from January to November 2021. Fresh breast tumor tissues were collated and homogenized for intracellular biochemical analysis using the enzyme-linked immunosorbent assay method. In total, 44 (58%) out of 65 patients, in the age range of 18-42 years and the mean±SD age of 32.55±6.40 years, had fibroadenomas, and other 21 (42%) cases, in the age range of 32-80 years and the mean±SD age of 56±14.4 years had invasive ductal carcinoma (IDC) breast cancer. Intracellular levels of HIF-1α, p53, and E2 were elevated significantly (<0.001) in IDC cases compared to the benign group. The most malignant tumors of IDC cases were in grade III and sizes T and T. The tissue concentrations of HIF-1α, P53, and E2 were significantly elevated in patients with tumor stage T3 compared to T and T. A significant elevation was found in the levels of HIF-1α, p53, and E2 in the positive LNM subgroup compared to the negative LNM group. Based on the obtained results, the prognostic value of the intracellular HIF-1α is considered to be a useful prognostic factor in Iraqi women with ICD and the combination of a HIF-1α protein with the nonfunctional p53 and E2 tends to indicate the proliferation, invasiveness, and metastases of the breast tumors.
乳腺癌是影响女性健康最常见的恶性肿瘤，全球发病率呈上升趋势。本研究旨在测量成年女性乳腺癌肿瘤组织中缺氧诱导因子 1α（HIF-1α）、肿瘤抑制蛋白 p53 和雌二醇（E2）的细胞内浓度，并探讨其与肿瘤分级、肿瘤大小和淋巴结转移（LNM）的关系。该研究于 2021 年 1 月至 11 月在伊拉克纳西里耶的侯赛因教学医院和哈比比教学医院对 65 名因乳腺肿块入院的成年女性患者进行，采用酶联免疫吸附试验方法对新鲜乳腺肿瘤组织进行细胞内生化分析。共有 44 名（58%）患者，年龄在 18-42 岁，平均年龄为 32.55±6.40 岁，患有纤维腺瘤；21 名（42%）患者，年龄在 32-80 岁，平均年龄为 56±14.4 岁，患有浸润性导管癌（IDC）乳腺癌。与良性组相比，IDC 病例的 HIF-1α、p53 和 E2 细胞内水平显著升高（<0.001）。IDC 病例中最恶性的肿瘤为 III 级和 T 和 T 期。与 T 和 T 相比，T3 期肿瘤患者的 HIF-1α、P53 和 E2 组织浓度显著升高。与 LNM 阴性组相比，LNM 阳性亚组的 HIF-1α、p53 和 E2 水平显著升高。根据所得结果，细胞内 HIF-1α 的预后价值被认为是伊拉克 IDC 女性患者的有用预后因素，HIF-1α 蛋白与非功能性 p53 和 E2 的结合倾向于表明乳腺肿瘤的增殖、侵袭和转移。
