Department of Urology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200127, China.
Department of Pathology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200127, China.
Med Oncol. 2023 Mar 8;40(4):113. doi: 10.1007/s12032-023-01977-z.
Previous studies have suggested the potential diagnostic value of circulating tumor cells (CTCs). This study aims to validate the efficacy of in vivo detection of CTCs in bladder cancer (BC) patients. A total of 216 BC patients were enrolled in this study. All patients had one in vivo detection of CTCs before initial treatment as a baseline parameter. The results of CTCs were associated with different clinicopathological features including molecular subtypes. PD-L1 expression on CTCs was also assessed and compared with its expression on tumors. CTC positive was defined as > 2 CTCs detected. Among all 216 patients, 49 (23%) were detected as CTC positive (> 2 CTCs) at baseline. Positive detection of CTCs was associated with multiple high-risk clinicopathological features including the multiplicity of the tumor (P = 0.02), tumor size (P < 0.01), tumor stage (P < 0.01), tumor grade (P < 0.01) and tumor PD-L1 expression (P = 0.01). The expression of PD-L1 on tumor and CTCs were not coordinated. Only 55% (74/134) matched the same status of PD-L1 expression on tumor and CTCs, along with 56 CTC Tissue and 4 CTC Tissue (P < 0.01). Our study has demonstrated the efficacy of in vivo detection of CTCs. The positive detection of CTCs is associated with multiple clinicopathological features. PD-L1 expression on CTCs has the potential to be a supplementary biomarker for immunotherapy.
先前的研究表明循环肿瘤细胞（CTC）具有潜在的诊断价值。本研究旨在验证体内检测膀胱癌（BC）患者CTC 的疗效。共有 216 例 BC 患者入组本研究。所有患者在初始治疗前均进行了一次体内 CTC 检测作为基线参数。CTC 结果与包括分子亚型在内的不同临床病理特征相关。还评估了 CTC 上 PD-L1 的表达并与其肿瘤上的表达进行了比较。CTC 阳性定义为检测到>2 个 CTC。在所有 216 例患者中，有 49 例（23%）在基线时被检测为 CTC 阳性（>2 个 CTC）。阳性 CTC 检测与多种高危临床病理特征相关，包括肿瘤的多发性（P=0.02）、肿瘤大小（P<0.01）、肿瘤分期（P<0.01）、肿瘤分级（P<0.01）和肿瘤 PD-L1 表达（P=0.01）。肿瘤和 CTC 上 PD-L1 的表达并不协调。只有 55%（74/134）的肿瘤和 CTC 上的 PD-L1 表达相同，同时还有 56 个 CTC 组织和 4 个 CTC 组织（P<0.01）。我们的研究证明了体内检测 CTC 的疗效。CTC 的阳性检测与多种临床病理特征相关。CTC 上 PD-L1 的表达有可能成为免疫治疗的补充生物标志物。
