Rui H, Brekke I, Mørkås L, Purvis K
Institute of Pathology, Rikshospitalet, Oslo/Norway.
Andrologia. 1987 Mar-Apr;19(2):134-42. doi: 10.1111/j.1439-0272.1987.tb01872.x.
The ventral and dorsal lobes of the rat prostate contain larger quantities of the aliphatic amines putrescine, spermidine and spermine and higher activities of the enzyme ornithine decarboxylase (ODC; EC 4.1.1.17) than other accessory sex glands. In contrast, the coagulating glands and the seminal vesicles contain only small quantities of the amines but the highest activities of the arginase (ARG; EC 3.5.3.1). Lineweaver-Burk plots indicated that the Km-values for ARG in the coagulating gland and ODC in the ventral prostate lobe were 20 mM and 0.2 mM, respectively. Castration decreased ODC and ARG activities to 3 and 50% of control levels, respectively, after 3 days, whilst the Km-values were unaffected. Daily administration of 3 mg dihydrotestosterone (DHT) prevented these castrational changes. Oestrogen treatment alone had no effect on the activities of the enzymes, but appeared to exert a synergistic effect with androgen on the ODC. Administration of androgen to intact rats for 7 days caused a dose-related alteration in the ratios of the various amines, particularly the spermine: putrescine ratio. A minor but significant decrease was also recorded in the activity of the ODC, which was mirrored by an increase in the levels of putrescine in the tissue. The data suggest that androgen control of the polyamine pathway is biphasic, first stimulatory and later inhibitory with lesions occurring at the ODC, possibly via short loop feedback of its product putrescine, but also at subsequent enzymic steps in spermidine and spermine biosynthesis.
大鼠前列腺的腹叶和背叶所含的脂肪族胺（腐胺、亚精胺和精胺）量比其他附属性腺多，鸟氨酸脱羧酶（ODC；EC 4.1.1.17）的活性也更高。相比之下，凝固腺和精囊仅含有少量的胺，但精氨酸酶（ARG；EC 3.5.3.1）的活性最高。Lineweaver-Burk图表明，凝固腺中ARG的Km值和前列腺腹叶中ODC的Km值分别为20 mM和0.2 mM。去势3天后，ODC和ARG的活性分别降至对照水平的3%和50%，而Km值不受影响。每天给予3 mg二氢睾酮（DHT）可防止这些去势引起的变化。单独雌激素处理对酶的活性没有影响，但似乎与雄激素对ODC有协同作用。对完整大鼠给予雄激素7天导致各种胺的比例发生剂量相关的改变，尤其是精胺：腐胺的比例。ODC的活性也有轻微但显著的降低，组织中腐胺水平的升高反映了这一点。数据表明，雄激素对多胺途径的控制是双相的，首先是刺激作用，随后是抑制作用，病变发生在ODC，可能是通过其产物腐胺的短环反馈，但也发生在亚精胺和精胺生物合成的后续酶促步骤中。
