College of Environmental Science & Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu Province, 224051, China.
China MCC5 Group Limited Corporation, Chengdu, 610023, China.
Chemosphere. 2023 May;324:138328. doi: 10.1016/j.chemosphere.2023.138328. Epub 2023 Mar 6.
Disgusting fishy odor could break out inside oligotrophic drinking waterbody in winter with low temperature. Nevertheless, fishy odor-producing algae and corresponding fishy odorants were not very clear, odor contribution of fishy odorant and odor-producing algae to overall odor profile were also not well understood. In this study, the fishy odorants, produced by four algae separated from Yanlong Lake, were identified simultaneously. Odor contribution of identified odorant, separated algae to overall fishy odor profile were both evaluated. The results indicated Yanlong Lake was mainly associated with fishy odor (flavor profile analysis (FPA) intensity: 6), eight, five, five and six fishy odorants were identified and determined in Cryptomonas ovate, Dinobryon sp., Synura uvella, Ochromonas sp., respectively, which were separated and cultured from water source. Totally sixteen odorants with concentration range of 90-880 ng/L, including hexanal, heptanal, 2,4-heptadienal, 1-octen-3-one, 1-octen-3-ol, octanal, 2-octenal, 2,4-octadienal, nonanal, 2-nonenal, 2,6-nonadienal, decanal, 2-decenal, 2,4-decadienal, undecanal, 2-tetradecanone, were verified in separated algae and associated with fishy odor. Although more odorants' odor activity value (OAV) were lower than one, approximately 89%, 91%, 87%, 90% of fishy odor intensities could be explained by reconstituting identified odorants for Cryptomonas ovate, Dinobryon sp., Synura uvella, Ochromonas sp., respectively, suggesting synergistic effect could exist among identified odorants. By calculating and evaluating total odorant production, total odorant OAV and cell odorant yield of separated algae, odor contribution rank to overall fishy odor should be Cryptomonas ovate (28.19%), Dinobryon sp. (27.05%), Synura uvella (24.27%), Ochromonas sp. (20.49%). This is the first study for identifying fishy odorants from four actually separated odor-producing algae simultaneously, this is also for the first time evaluating and explaining odor contribution of identified odorant, separated algae to overall odor profile comprehensively, this study will supply more understanding for controlling and managing fishy odor in drinking water treatment plant.
冬季低温寡营养型饮用水体内可能会突然散发出令人作呕的鱼腥气味。然而，鱼腥气味产生藻类和相应的鱼腥气味物质并不十分清楚，鱼腥气味物质和产生藻类对整体气味特征的贡献也不是很了解。本研究同时鉴定了从延龙湖水体中分离得到的四种藻类产生的鱼腥气味物质。评价了鉴定出的气味物质、分离出的藻类对整体鱼腥气味特征的贡献。结果表明，延龙湖主要与鱼腥气味（风味特征分析（FPA）强度：6）有关，从水源中分离培养的卵形隐藻、Dino-bryon 属、Synura uvella、Ochromonas 属分别鉴定并确定了八种、五种、五种和六种鱼腥气味物质。共鉴定出十六种浓度范围为 90-880ng/L 的气味物质，包括己醛、庚醛、2,4-庚二烯醛、1-辛烯-3-酮、1-辛烯-3-醇、辛醛、2-辛烯醛、2,4-辛二烯醛、壬醛、2-壬烯醛、2,6-壬二烯醛、癸醛、2-癸烯醛、2,4-癸二烯醛、十一醛、2-十四烷酮，这些物质存在于分离的藻类中，并与鱼腥气味有关。尽管更多气味物质的气味活性值（OAV）低于 1，但分别用鉴定出的气味物质重构卵形隐藻、Dino-bryon 属、Synura uvella、Ochromonas 属的鱼腥气味强度时，约有 89%、91%、87%、90%的鱼腥气味强度可以得到解释，这表明鉴定出的气味物质之间可能存在协同作用。通过计算和评估分离藻类的总气味物质产生量、总气味物质 OAV 和细胞气味物质产率，藻类对整体鱼腥气味的贡献排序应为卵形隐藻（28.19%）、Dino-bryon 属（27.05%）、Synura uvella（24.27%）、Ochromonas 属（20.49%）。这是首次同时鉴定四种实际分离的产鱼腥气味藻类的鱼腥气味物质，也是首次全面评价和解释鉴定出的气味物质、分离的藻类对整体气味特征的贡献，这将为饮用水处理厂控制和管理鱼腥气味提供更多的了解。
