Imran Iqbal Bin, Engström Marica T, Karonen Maarit, Williams Andrew R, Salminen Juha-Pekka
Natural Chemistry Research Group, Department of Chemistry, University of Turku, FI-20014, Turku, Finland; Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark.
Natural Chemistry Research Group, Department of Chemistry, University of Turku, FI-20014, Turku, Finland.
Exp Parasitol. 2023 May;248:108493. doi: 10.1016/j.exppara.2023.108493. Epub 2023 Mar 6.
Proanthocyanidins (PAs) are a class of plant specialized metabolites with well-documented bioactivities such as antiparasitic effects. However, little is known about how the modification of PAs influences their bioactivity. The objective of this study was to investigate a wide range of PA-containing plant samples to determine if extracts containing PAs modified by oxidation had altered antiparasitic activities, compared to the original extracts that had not been modified in alkaline conditions. We extracted and analyzed samples from 61 proanthocyanidin-rich plants. The extracts were then oxidized under alkaline conditions. We used these non-oxidized and oxidized proanthocyanidin-rich extracts to conduct a detailed analysis of direct antiparasitic effects against the intestinal parasite Ascaris suum in vitro. These tests showed that the proanthocyanidin-rich extracts had antiparasitic activity. Modification of these extracts significantly increased the antiparasitic activity for the majority the extracts, suggesting that the oxidation procedure enhanced the bioactivity of the samples. Some samples that showed no antiparasitic activity before oxidation showed very high activity after the oxidation. High levels of other polyphenols in the extracts, such as flavonoids, was found to be associated with increased antiparasitic activity following oxidation. Thus, our in vitro screening opens up the opportunity for future research to better understand the mechanism of action how alkaline treatment of PA-rich plant extracts increases their biological activity and potential as novel anthelmintics.
原花青素（PAs）是一类植物特化代谢产物，具有如抗寄生虫作用等诸多已被充分证明的生物活性。然而，关于原花青素的修饰如何影响其生物活性却知之甚少。本研究的目的是调查一系列含原花青素的植物样本，以确定与在碱性条件下未经过修饰的原始提取物相比，含有经氧化修饰的原花青素的提取物是否具有改变的抗寄生虫活性。我们从61种富含原花青素的植物中提取并分析了样本。然后将提取物在碱性条件下进行氧化。我们使用这些未氧化和氧化的富含原花青素的提取物，对体外针对肠道寄生虫猪蛔虫的直接抗寄生虫作用进行了详细分析。这些测试表明，富含原花青素的提取物具有抗寄生虫活性。对这些提取物的修饰显著提高了大多数提取物的抗寄生虫活性，这表明氧化过程增强了样本的生物活性。一些在氧化前没有抗寄生虫活性的样本在氧化后显示出非常高的活性。提取物中高水平的其他多酚类物质，如黄酮类化合物，被发现与氧化后抗寄生虫活性的增加有关。因此，我们的体外筛选为未来的研究提供了机会，以便更好地理解富含原花青素的植物提取物经过碱性处理后增加其生物活性及作为新型驱虫剂潜力的作用机制。
