贝克曼分析仪检测甲状腺功能时生物素的显著干扰：如何识别这种干扰？
Significant Interference of Biotin in Thyroid Function Tests Using Beckman Analyzer: How to Identify such Interferences?
机构信息
Department of Clinical Laboratory Sciences, University of Kansas Medical Center, Kansas City, KS, USA.
Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS, USA
出版信息
Ann Clin Lab Sci. 2023 Jan;53(1):130-133.
OBJECTIVE
Biotin in elevated concentration interferes with biotin-based immunoassays. We studied biotin interferences in TSH, FT4, FT3, total T4, total T3 and thyroglobulin assays both and using Beckman DXI800 analyzer.
METHODS
Two serum pools were prepared from left-over specimens. Then aliquots of each pool (and serum control) were supplemented with various amounts of biotin followed by measuring thyroid function tests again. Three volunteers each took 10 mg biotin supplement. We compared thyroid function tests before and 2 h after taking biotin.
RESULTS
We observed significant biotin interferences in biotin-based assays (positive interference with FT4, FT3, and total T3 assay but negative interference with thyroglobulin) both in vitro and in vivo but non-biotin-based assays (TSH and total T4) were not affected.
CONCLUSIONS
Elevated FT3 and FT4 in the presence of normal TSH is inconsistent with hyperthyroidism and should be followed up with total T3 and T4 test. Significant discrepancy between total T3 (falsely elevated value due to biotin) and total T4 (not affected as the assay is not biotin based) maybe an indication of biotin interference.
目的
高浓度的生物素会干扰基于生物素的免疫分析。我们研究了贝克曼 DXI800 分析仪上 TSH、FT4、FT3、总 T4、总 T3 和甲状腺球蛋白检测中的生物素干扰。
方法
从剩余标本中制备了两个血清池。然后将每个池的等分试样（和血清对照）用各种量的生物素补充，然后再次测量甲状腺功能测试。每个志愿者服用 10 毫克生物素补充剂。我们比较了服用生物素前后 2 小时的甲状腺功能测试。
结果
我们观察到体外和体内基于生物素的检测中存在显著的生物素干扰（FT4、FT3 和总 T3 检测呈阳性干扰，但甲状腺球蛋白检测呈阴性干扰），但非基于生物素的检测（TSH 和总 T4）不受影响。
结论
在 TSH 正常的情况下，FT3 和 FT4 升高与甲亢不一致，应随访总 T3 和 T4 检测。总 T3（由于生物素而导致的假高值）和总 T4（不受影响，因为该检测不是基于生物素）之间的显著差异可能表明存在生物素干扰。
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