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香芹酚通过破坏细胞壁的完整性和组成来抑制导致枸杞果实腐烂的作用。

Inhibitory effect of carvacrol against causing goji fruit rot by disrupting the integrity and composition of cell wall.

作者信息

Zhao Lunaike, Wang Junjie, Zhang Huaiyu, Wang Peng, Wang Cong, Zhou Yueli, Li Huanhuan, Yu Shukun, Wu Rina

机构信息

Key Laboratory of Storage and Processing of Plant Agro-Products, School of Biological Science and Engineering, North Minzu University, Yinchuan, China.

出版信息

Front Microbiol. 2023 Feb 20;14:1139749. doi: 10.3389/fmicb.2023.1139749. eCollection 2023.

DOI:10.3389/fmicb.2023.1139749
PMID:36891390
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9986456/
Abstract

Goji ( L.) is a widely planted crop in China that is easily infected by the pathogenic fungus , which causes rot after harvest. Previous studies showed that carvacrol (CVR) significantly inhibited the mycelial growth of and reduced Alternaria rot in goji fruits . The present study aimed to explore the antifungal mechanism of CVR against . Optical microscopy and calcofluor white (CFW) fluorescence observations showed that CVR affected the cell wall of . CVR treatment affected the integrity of the cell wall and the content of substances in the cell wall as measured by alkaline phosphatase (AKP) activity, Fourier transform-infrared spectroscopy (FT-IR), and X-ray photoelectron spectroscopy (XPS). Chitin and β-1,3-glucan contents in cells decreased after CVR treatment, and the activities of β-glucan synthase and chitin synthase decreased. Transcriptome analysis revealed that CVR treatment affected cell wall-related genes in , thereby affecting cell wall growth. Cell wall resistance also decreased with CVR treatment. Collectively, these results suggest that CVR may exert antifungal activity by interfering with cell wall construction, leading to impairment of cell wall permeability and integrity.

摘要

枸杞（Lycium barbarum L.）是中国广泛种植的作物，易受致病真菌感染，导致采后腐烂。先前的研究表明，香芹酚（CVR）能显著抑制该致病真菌的菌丝生长，并减少枸杞果实的链格孢属腐烂病。本研究旨在探究CVR对该致病真菌的抗真菌机制。光学显微镜和荧光增白剂（CFW）荧光观察表明，CVR影响该致病真菌的细胞壁。通过碱性磷酸酶（AKP）活性、傅里叶变换红外光谱（FT-IR）和X射线光电子能谱（XPS）测定，CVR处理影响细胞壁的完整性和细胞壁中物质的含量。CVR处理后，细胞中的几丁质和β-1,3-葡聚糖含量降低，β-葡聚糖合酶和几丁质合酶的活性降低。转录组分析表明，CVR处理影响该致病真菌中与细胞壁相关的基因，从而影响细胞壁生长。CVR处理也降低了细胞壁抗性。总体而言，这些结果表明，CVR可能通过干扰细胞壁构建发挥抗真菌活性，导致细胞壁通透性和完整性受损。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac7e/9986456/6fb24a9b90dd/fmicb-14-1139749-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac7e/9986456/8c709d43d411/fmicb-14-1139749-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac7e/9986456/972919b04b00/fmicb-14-1139749-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac7e/9986456/62ff219adeb0/fmicb-14-1139749-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac7e/9986456/af0032398623/fmicb-14-1139749-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac7e/9986456/42dfd32f7056/fmicb-14-1139749-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac7e/9986456/6fb24a9b90dd/fmicb-14-1139749-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac7e/9986456/8c709d43d411/fmicb-14-1139749-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac7e/9986456/972919b04b00/fmicb-14-1139749-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac7e/9986456/62ff219adeb0/fmicb-14-1139749-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac7e/9986456/af0032398623/fmicb-14-1139749-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac7e/9986456/42dfd32f7056/fmicb-14-1139749-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac7e/9986456/6fb24a9b90dd/fmicb-14-1139749-g006.jpg

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