Suzuki M, Harada Y, Hirakawa H, Hirakawa K, Omura R
Department of Otolaryngology, Hiroshima University, School of Medicine, Japan.
Arch Otorhinolaryngol. 1987;244(4):215-7. doi: 10.1007/BF00455308.
The frog Rana nigromaculata was used as an experimental model. The utricle and its nerve were isolated with the anterior and the lateral semicircular canal ampullae in frog Ringer's solution. The utricular otoconia and the otoconial membrane were then carefully removed. The halved anterior canal cupula was next placed in the tip of a glass microelectrode, which was mounted on a micromanipulator. The cupula was placed in the center of either the medial or the lateral part of the macula and was moved toward or away from the striola along the axis vertical to the striola. When the medial part of the macula was stimulated, 5 micron of striolapetal cupular movement elicited an excitatory nerve discharge. When the lateral part of the macula was stimulated, the striolapetal stimulus likewise induced an excitatory response. These results indicate the existence of physiological polarity on the utricular macula.
黑斑蛙被用作实验模型。在蛙任氏液中，将椭圆囊及其神经与前半规管和外侧半规管壶腹一起分离出来。然后小心地去除椭圆囊耳石和耳石膜。接下来，将切成两半的前半规管壶腹放置在安装在显微操作器上的玻璃微电极尖端。将壶腹放置在椭圆囊斑内侧或外侧部分的中心，并沿着与纹状区垂直的轴朝向或远离纹状区移动。当刺激椭圆囊斑的内侧部分时，向纹状区方向5微米的壶腹运动引发兴奋性神经放电。当刺激椭圆囊斑的外侧部分时，向纹状区方向的刺激同样诱发兴奋性反应。这些结果表明椭圆囊斑上存在生理极性。
