Department of Environmental Science and Engineering, College of Environment and Resources, Xiangtan University, Xiangtan, China.
School of Metallurgy and Environment, Central South University, Changsha, China.
Biotechnol Bioeng. 2023 Jun;120(6):1557-1568. doi: 10.1002/bit.28373. Epub 2023 Mar 18.
Lignin separation from natural lignocellulose for the preparation of lignin nanoparticles (LNPs) is often challenging owing to the recalcitrant and complex structure of lignocellulose. This paper reports a strategy for the rapid synthesis of LNPs via microwave-assisted lignocellulose fractionation using ternary deep eutectic solvents (DESs). A novel ternary DES with strong hydrogen bonding was prepared using choline chloride, oxalic acid, and lactic acid in a 1:0.5:1 ratio. Efficient fractionation of rice straw (0.5 × 2.0 cm) (RS) was realized by the ternary DES under microwave irradiation (680 W) within only 4 min, and 63.4% of lignin could be separated from the RS to prepare LNPs with a high lignin purity (86.8%), an average particle size of 48-95 nm, and a narrow size distribution. The mechanism of lignin conversion was also investigated, which revealed that dissolved lignin aggregated into LNPs via π-π stacking interactions.
由于木质纤维素具有顽固和复杂的结构，因此从天然木质纤维素中分离木质素以制备木质素纳米粒子（LNP）通常具有挑战性。本文报道了一种通过使用三元深共熔溶剂（DES）进行微波辅助木质纤维素分级来快速合成 LNP 的策略。使用氯化胆碱、草酸和乳酸以 1:0.5:1 的比例制备了具有强氢键的新型三元 DES。通过三元 DES 在微波辐射（680 W）下仅 4 分钟即可实现稻秸秆（0.5×2.0 cm）（RS）的有效分级，并且可以从 RS 中分离出 63.4%的木质素来制备具有高木质素纯度（86.8%）的 LNP，平均粒径为 48-95nm，粒径分布较窄。还研究了木质素转化的机理，结果表明溶解的木质素通过π-π堆积相互作用聚集形成 LNP。
