Department of Plant Science and Landscape Architecture, University of Maryland College Park, MD 20742, U.S.A.
Institute for Bioscience and Biotechnology Research, Rockville, MD 20850, U.S.A.
Mol Plant Microbe Interact. 2023 Aug;36(8):489-501. doi: 10.1094/MPMI-12-22-0247-R. Epub 2023 Jun 10.
Fusarium head blight (FHB), caused by the hemibiotrophic fungus , is one of the major threats to global wheat productivity. A wheat pore-forming toxin-like (PFT) protein was previously reported to underlie , the most widely used quantitative trait locus in FHB breeding programs worldwide. In the present work, wheat PFT was ectopically expressed in the model dicot plant . Heterologous expression of wheat PFT in provided a broad-spectrum quantitative resistance to fungal pathogens including , , , and . However, there was no resistance to bacterial or oomycete pathogens and , respectively in the transgenic plants. To explore the reason for the resistance response to, exclusively, the fungal pathogens, purified PFT protein was hybridized to a glycan microarray having 300 different types of carbohydrate monomers and oligomers. It was found that PFT specifically hybridized with chitin monomer, -acetyl glucosamine (GlcNAc), which is present in fungal cell walls but not in bacteria or oomycete species. This exclusive recognition of chitin may be responsible for the specificity of PFT-mediated resistance to fungal pathogens. Transfer of the atypical quantitative resistance of wheat PFT to a dicot system highlights its potential utility in designing broad-spectrum resistance in diverse host plants. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
镰刀菌穗枯病（FHB）由半活体真菌引起，是全球小麦生产力的主要威胁之一。先前有报道称，小麦形成孔蛋白样（PFT）蛋白是 FHB 育种计划中使用最广泛的数量性状位点之一。在本工作中，将小麦 PFT 异位表达在模式双子叶植物 中。在 中异源表达小麦 PFT 为包括 、 、 和 在内的真菌病原体提供了广谱定量抗性。然而，在转基因 植物中，对细菌或卵菌病原体 和 没有抗性。为了探索对真菌病原体 表现出抗性反应的原因，仅对真菌病原体，纯化的 PFT 蛋白与包含 300 种不同类型糖单体和寡聚物的糖基微阵列杂交。结果发现 PFT 特异性地与真菌细胞壁中存在但在细菌或卵菌物种中不存在的几丁质单体、N-乙酰葡萄糖胺（GlcNAc）杂交。这种几丁质的特异性识别可能是 PFT 介导的对真菌病原体抗性特异性的原因。将小麦 PFT 的非典型数量抗性转移到双子叶系统中，突出了其在设计不同宿主植物广谱抗性方面的潜在用途。[公式：见正文]版权所有 © 2023 作者。这是一个 CC BY-NC-ND 4.0 国际许可下的开放获取文章。
