Graduate School of Health and Sports Science, Juntendo University, Inzai, Chiba, JAPAN.
Faculty of Sport and Health Science, Ritsumeikan University, Kusatsu, Shiga, JAPAN.
Med Sci Sports Exerc. 2023 Jul 1;55(7):1184-1194. doi: 10.1249/MSS.0000000000003151. Epub 2023 Feb 24.
A ketone body (β-hydroxybutyrate [β-HB]) is used as an energy source in the peripheral tissues. However, the effects of acute β-HB supplementation on different modalities of exercise performance remain unclear. This study aimed to assess the effects of acute β-HB administration on the exercise performance of rats.
In study 1, Sprague-Dawley rats were randomly divided into six groups: endurance exercise (EE + PL and EE + KE), resistance exercise (RE + PL and RE + KE), and high-intensity intermittent exercise (HIIE + PL and HIIE + KE) with placebo (PL) or β-HB salt (KE) administration. In study 2, metabolome analysis using capillary electrophoresis mass spectrometry was performed to profile the effects of β-HB salt administration on HIIE-induced metabolic responses in the skeletal and heart muscles.
The maximal carrying capacity (rest for 3 min after each ladder climb, while carrying heavy weights until the rats could not climb) in the RE + KE group was higher than that in the RE + PL group. The maximum number of HIIE sessions (a 20-s swimming session with a 10-s rest between sessions, while bearing a weight equivalent to 16% of body weight) in the HIIE + KE group was higher than that in the HIIE + PL group. However, there was no significant difference in the time to exhaustion at 30 m·min -1 between the EE + PL and the EE + KE groups. Metabolome analysis showed that the overall tricarboxylic acid cycle and creatine phosphate levels in the skeletal muscle were higher in the HIIE + KE group than those in the HIIE + PL group.
These results indicate that acute β-HB salt administration may accelerate HIIE and RE performance, and the changes in metabolic responses in the skeletal muscle after β-HB salt administration may be involved in the enhancement of HIIE performance.
酮体（β-羟丁酸[β-HB]）可用作外周组织的能量来源。然而，急性β-HB 补充对不同运动方式的运动表现的影响尚不清楚。本研究旨在评估急性β-HB 给药对大鼠运动表现的影响。
在研究 1 中，将 Sprague-Dawley 大鼠随机分为六组：耐力运动（EE+PL 和 EE+KE）、抗阻运动（RE+PL 和 RE+KE）和高强度间歇运动（HIIE+PL 和 HIIE+KE），给予安慰剂（PL）或β-HB 盐（KE）。在研究 2 中，使用毛细管电泳质谱法进行代谢组学分析，以分析β-HB 盐给药对 HIIE 诱导的骨骼肌和心肌代谢反应的影响。
在 RE+KE 组中，最大承重能力（每次爬梯后休息 3 分钟，同时携带重物直到大鼠无法再爬）高于 RE+PL 组。在 HIIE+KE 组中，最大 HIIE 次数（20 秒游泳，每次休息 10 秒，同时负重相当于体重的 16%）高于 HIIE+PL 组。然而，在 30 m·min -1 时，EE+PL 和 EE+KE 组之间的力竭时间没有显著差异。代谢组学分析显示，HIIE+KE 组骨骼肌中的三羧酸循环和磷酸肌酸水平总体较高。
这些结果表明，急性β-HB 盐给药可能加速 HIIE 和 RE 表现，并且β-HB 盐给药后骨骼肌代谢反应的变化可能参与了 HIIE 表现的增强。
