Department of Animal Science and Technology, Chung-Ang University, Anseong, Korea.
Department of Veterinary Microbiology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea.
Foodborne Pathog Dis. 2023 Mar;20(3):100-109. doi: 10.1089/fpd.2022.0076.
High prevalence of livestock-associated methicillin-susceptible and -resistant (LA-MSSA and LA-MRSA, respectively) in livestock farms, particularly pig farms, is an increasingly serious threat to food safety and public health. In this study, 173 (84 MRSA and 89 MSSA) isolates from healthy pigs, farm environments, and farm workers in Korea were examined to determine the (1) genetic diversity of isolates (sequence type [ST], , and types), (2) staphylococcal cassette chromosome (SCC) types of MRSA isolates, and (3) multidrug resistance (MDR) phenotypes of MRSA and MSSA isolates. Clonal complex 398 (CC398) genotypes of MRSA and MSSA isolates, particularly CC398- type t571- I lineages, displaying MDR phenotypes were highly prevalent in pig farms. High prevalence of CC398-t571 MRSA and MSSA was more frequently associated with weaning piglets and growing pigs. Moreover, the same clonal lineages of isolates colonized both pigs and farm workers, suggesting the transmission of antimicrobial-resistant CC398 MRSA and MSSA between pigs and humans in the pig farms. Furthermore, two dominant SCC types, SCC V and SCC IX, were identified in CC398 MRSA isolates colonizing healthy pigs. To the best of our knowledge, this is the first report of a CC398 LA-MRSA isolate carrying SCC IX in Korea. Collectively, these results suggest widespread distribution of the CC398 lineage among MRSA and MSSA isolates in pigs, farm environments, and farm workers in Korea.
高流行率的牲畜相关的耐甲氧西林金黄色葡萄球菌（LA-MSSA 和 LA-MRSA，分别）在农场的牲畜，尤其是养猪场，是一个日益严重的威胁，食品安全和公众健康。在这项研究中，173 （84 株耐甲氧西林金黄色葡萄球菌和 89 MSSA）分离株从健康猪，农场环境和农场工人在韩国被检查，以确定（1）遗传多样性的分离株（序列类型[ST]，和类型），（2）耐甲氧西林金黄色葡萄球菌分离株的葡萄球菌盒染色体（SCC）类型，和（3）耐甲氧西林金黄色葡萄球菌和 MSSA 分离株的多药耐药（MDR）表型。MRSA 和 MSSA 分离株的克隆复合体 398（CC398）基因型，特别是 CC398-类型 t571-我谱系，表现出多药耐药表型，在养猪场中非常普遍。CC398-t571 耐甲氧西林金黄色葡萄球菌和 MSSA 的高流行率与断奶仔猪和生长猪更频繁地相关。此外，相同的克隆谱系的分离株定植于猪和农场工人，表明在养猪场中耐抗菌药物抗性 CC398 MRSA 和 MSSA 之间的猪与人之间的传播。此外，两种主要的 SCC 类型，SCC V 和 SCC IX，在定植于健康猪的 CC398 耐甲氧西林金黄色葡萄球菌分离株中被鉴定出来。据我们所知，这是在韩国首次报道携带 SCCIX 的 CC398 型 LA-MRSA 分离株。总之，这些结果表明在韩国的猪、农场环境和农场工人中，CC398 谱系在耐甲氧西林金黄色葡萄球菌和 MSSA 分离株中广泛分布。
