Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221, USA; Department of Chemistry, Universidade Federal de São Paulo, Diadema, SP 09972-270, Brazil.
Department of Chemistry, Universidade Federal de São Paulo, Diadema, SP 09972-270, Brazil.
J Hazard Mater. 2023 Jun 5;451:131098. doi: 10.1016/j.jhazmat.2023.131098. Epub 2023 Feb 26.
Mushrooms may incorporate significant levels of Hg making its consumption harmful to human health. Mercury remediation induced by Se competition in edible mushrooms represents a valuable alternative since Se plays effective roles against Hg uptake, accumulation, and toxicity. In this way, Pleurotus ostreatus and Pleurotus djamor were cultivated on Hg-contaminated substrate simultaneously supplemented with Se(IV) or Se(VI) under different dosages in this study. The protective role of Se was assessed taking into account morphological characteristics and Hg and Se total concentrations determined by ICP-MS, as well as proteins and protein-bound Hg and Se distribution by SEC-UV-ICP-MS, and Hg speciation studies (Hg(II) and MeHg) by HPLC-ICP-MS. Both Se(IV) and Se(VI) supplementation were able to recover the morphology mainly of Hg-contaminated Pleurotus ostreatus. The mitigation effects induced by Se(IV) stood out more than Se(VI) in terms of Hg incorporation, decreasing the total Hg concentration up to 96 %. Also, it was found that supplementation mainly with Se(IV) reduced the fraction of Hg bound to medium molecular weight compounds (17-44 kDa) up to 80 %. Finally, it was shown a Se-induced inhibitory effect on Hg methylation, decreasing MeHg species content in mushrooms exposed to Se(IV) (51.2 µg g) up to 100 %.
蘑菇可能含有大量的汞，其食用对人类健康有害。硒对食用蘑菇中汞的修复作用是一种很有价值的替代方法，因为硒在抑制汞的吸收、积累和毒性方面发挥着有效的作用。在这项研究中，我们同时在受汞污染的基质上培养糙皮侧耳和美味侧耳，并在不同剂量下用硒（IV）或硒（VI）补充硒。通过 ICP-MS 测定的形态特征和总汞、总硒浓度，以及 SEC-UV-ICP-MS 测定的蛋白质和蛋白质结合的汞、硒分布，以及 HPLC-ICP-MS 测定的汞形态（Hg(II)和 MeHg），评估了硒的保护作用。硒（IV）和硒（VI）的补充都能够恢复主要受汞污染的糙皮侧耳的形态。与硒（VI）相比，硒（IV）的缓解效果更为明显，可将总汞浓度降低多达 96%。此外，研究发现，主要补充硒（IV）可将结合到中分子量化合物（17-44 kDa）的汞比例降低多达 80%。最后，结果表明，硒对汞甲基化有抑制作用，使暴露于硒（IV）的蘑菇中的 MeHg 含量（51.2 µg g）降低多达 100%。
