Liu Jiameng, Wang Mengfei, Guo Chuanpan, Tao Zheng, Wang Minghua, He Linghao, Liu Baozhong, Zhang Zhihong
College of Chemistry and Chemical Engineering, Henan Polytechnic University, 2001 Century Avenue, Jiaozuo 454000, PR China.
College of Material and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China.
Food Chem. 2023 Aug 1;416:135839. doi: 10.1016/j.foodchem.2023.135839. Epub 2023 Mar 5.
An electrochemical aptasensor was prepared for the efficient, sensitive, and selective detection of 17β-estradiol. The sensor was based on a defective two-dimensional porphyrin-based metal-organic framework derived from VCT MXene. The resulting metal-organic framework nanosheets benefited from the advantages of VCT MXene nanosheets and porphyrin-based metal-organic framework, two-dimensional porphyrin-based metal-organic framework nanosheets demonstrated amplified electrochemical response and enhanced aptamer-immobilization ability compared with VCT MXene nanosheets. The sensor's detection limit was ultralow at 0.81 fg mL (2.97 fM), and the 17β-estradiol concentration range was wide, thereby outperforming most reported aptasensors. The high selectivity, superior stability and reproducibility, and excellent regeneration performance of the constructed aptasensor indicated its remarkable potential application for 17β-estradiol determination in diverse real samples. This aptasensing strategy can be used to analyze other targets by replacing the corresponding aptamer.
制备了一种用于高效、灵敏且选择性检测17β-雌二醇的电化学适配体传感器。该传感器基于源自VCT MXene的缺陷二维卟啉基金属有机框架。所得的金属有机框架纳米片受益于VCT MXene纳米片和卟啉基金属有机框架的优点，与VCT MXene纳米片相比，二维卟啉基金属有机框架纳米片表现出放大的电化学响应和增强的适配体固定能力。该传感器的检测限超低，为0.81 fg/mL（2.97 fM），且17β-雌二醇的浓度范围很宽，从而优于大多数已报道的适配体传感器。所构建的适配体传感器具有高选择性、优异的稳定性和重现性以及出色的再生性能，表明其在多种实际样品中测定17β-雌二醇具有显著的潜在应用价值。通过替换相应的适配体，这种适配体传感策略可用于分析其他目标物。
