基于全长 SMRT 转录组测序的（Drury）对 Bizio 感染的转录组反应。

The transcriptomic response of (Drury) to the infection of Bizio based on full-length SMRT transcriptome sequencing.

机构信息

Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.

College of Forestry, Nanjing Forestry University, Nanjing, China.

出版信息

Front Cell Infect Microbiol. 2023 Feb 16;13:1093432. doi: 10.3389/fcimb.2023.1093432. eCollection 2023.

DOI:10.3389/fcimb.2023.1093432

PMID:36896191

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9989771/

Abstract

(Drury) is a globally important forest pest. We found that the Bizio strain SM1 had insecticidal activity against , but the transcriptomic response of to SM1 were not clear. Therefore, we performed full-length sequencing of the transcriptomes of larvae infected with SM1 and the control group. A total of 1,183 differentially expressed genes (DEGs) were identified by comparing the group infected with SM1 and the control group, including 554 downregulated genes and 629 upregulated genes. We found many downregulated genes in metabolic pathways. Furthermore, some of these downregulated genes were involved in cellular immunity, melanization, and detoxification enzymes, which showed that SM1 weakened immunity. In addition, genes in the juvenile hormone synthesis pathway were upregulated, which was detrimental to the survival of . This research analyzed the transcriptomic response of to SM1 by high-throughput full-length transcriptome sequencing. The results provide useful information to explore the relationship between and , and theoretical support for the application of and the control of in the future.

摘要

（Drury）是一种具有全球重要意义的森林害虫。我们发现 Bizio 菌株 SM1 对具有杀虫活性，但对 SM1 的转录组反应尚不清楚。因此，我们对感染 SM1 的和对照组的幼虫的全长转录组进行了测序。通过比较感染 SM1 的组和对照组，共鉴定出 1183 个差异表达基因（DEGs），包括 554 个下调基因和 629 个上调基因。我们在代谢途径中发现了许多下调基因。此外，其中一些下调基因参与细胞免疫、黑化和解毒酶，这表明 SM1 削弱了的免疫力。此外，昆虫激素合成途径中的基因上调，这对的生存不利。这项研究通过高通量全长转录组测序分析了对 SM1 的转录组反应。研究结果为探索与之间的关系提供了有用的信息，为未来和的应用以及的控制提供了理论支持。