Wei Xinju, Xu Danping, Liu Zhiqian, Liu Quanwei, Zhuo Zhihang
College of Life Science, China West Normal University, Nanchong 637002, China.
Insects. 2023 Jul 11;14(7):625. doi: 10.3390/insects14070625.
(Hope) (Coleoptera: Cerambycidae) is an important forest pest in China that mainly infests timber and economic forests. This pest primarily causes plant tissue to necrotize, rot, and eventually die by feeding on the woody parts of tree trunks. To gain a deeper understanding of the genetic mechanism of , this study employed single-molecule real-time sequencing (SMRT) and Illumina RNA-seq technologies to conduct full-length transcriptome sequencing of the insect. Total RNA extracted from male and female adults was mixed and subjected to SMRT sequencing, generating a complete transcriptome. Transcriptome analysis, prediction of long non-coding RNA (lncRNA), coding sequences (CDs), analysis of simple sequence repeats (SSR), prediction of transcription factors, and functional annotation of transcripts were performed in this study. The collective 20,356,793 subreads (38.26 G, clean reads) were generated, including 432,091 circular consensus sequences and 395,851 full-length non-chimera reads. The full-length non-chimera reads (FLNC) were clustered and redundancies were removed, resulting in 39,912 consensus reads. SSR and ANGEL software v3.0 were used for predicting SSR and CDs. In addition, four tools were used for annotating 6058 lncRNAs, identifying 636 transcription factors. Furthermore, a total of 84,650 transcripts were functionally annotated in seven different databases. This is the first time that the full-length transcriptome of has been obtained using SMRT sequencing. This provides an important foundation for investigating the gene regulation underlying the interaction between and its host plants through gene editing in the future and provides a scientific basis for the prevention and control of .
锈色粒肩天牛（鞘翅目：天牛科）是中国一种重要的森林害虫，主要危害用材林和经济林。这种害虫主要通过取食树干木质部分，导致植物组织坏死、腐烂，最终死亡。为了更深入了解锈色粒肩天牛的遗传机制，本研究采用单分子实时测序（SMRT）和Illumina RNA-seq技术对该昆虫进行全长转录组测序。从雌雄成虫中提取的总RNA混合后进行SMRT测序，生成了一个完整的转录组。本研究进行了转录组分析、长链非编码RNA（lncRNA）预测、编码序列（CDs）分析、简单序列重复（SSR）预测、转录因子预测以及转录本的功能注释。共产生了20356793条子序列（38.26 G，clean reads），包括432091个环形一致序列和395851个全长非嵌合 reads。对全长非嵌合 reads（FLNC）进行聚类并去除冗余，得到39912个一致 reads。使用SSR和ANGEL软件v3.0预测SSR和CDs。此外，使用四种工具对6058个lncRNAs进行注释，鉴定出636个转录因子。此外，共有84650个转录本在七个不同数据库中进行了功能注释。这是首次使用SMRT测序获得锈色粒肩天牛的全长转录组。这为未来通过基因编辑研究锈色粒肩天牛与其寄主植物相互作用的基因调控提供了重要基础，并为锈色粒肩天牛的防治提供了科学依据。
