Institute of Jeju Microbial Resources, BioPS Co., Ltd., Jeju 63243, Republic of Korea.
Department of Biological Sciences and Biotechnology, Hannam University, Daejon 34054, Republic of Korea.
Int J Syst Evol Microbiol. 2023 Mar;73(3). doi: 10.1099/ijsem.0.005729.
Two Gram-positive bacterial strains, designated as YMB-B2 and BWT-G7, were isolated from larvae of L. and , respectively, and their taxonomic positions examined by a polyphasic approach. Both of the isolates contained ornithine as the cell-wall diamino acid. The acyl type of murein was -glycolyl. The predominant menaquinones were MK-11 and MK-12. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid. Both of the isolates contained C anteiso and C anteiso as the major fatty acids. Strain YMB-B2 also had C iso as an additional major fatty acid. The 16S rRNA gene phylogeny showed that the novel strains formed two distinct sublines within the genus . Strain YMB-B2 was most closely related to the type strains of (99.1 % sequence similarity) and (99.0 %) whereas strain BWT-G7 formed a tight cluster with the type strain of (98.9 %). The phylogenomic analysis based on 92 core genes supported their relationships in 16S rRNA gene phylogeny. Overall genomic relatedness indices warranted that the isolates represent two new species of the genus . Based on the results obtained here, sp. nov. (type strain YMB-B2=KCTC 49593=CCM 9151) and sp. nov. (type strain BWT-G7=KACC 22262=NBRC 115127) are proposed.
两株革兰氏阳性细菌菌株，分别命名为 YMB-B2 和 BWT-G7，分别从 L. 和 幼虫中分离得到，并通过多相方法研究了它们的分类地位。这两个分离株都含有鸟氨酸作为细胞壁二氨基庚二酸。壁酰类型为 - 甘氨酰。主要的menaquinones 是 MK-11 和 MK-12。极性脂是双磷脂酰甘油、磷脂酰甘油和一种未鉴定的糖脂。这两个分离株都含有 C anteiso 和 C anteiso 作为主要脂肪酸。菌株 YMB-B2 还含有 C iso 作为额外的主要脂肪酸。16S rRNA 基因系统发育表明，这两个新菌株在属内形成了两个不同的亚系。菌株 YMB-B2 与 （99.1%序列相似性）和 （99.0%）的模式菌株最为密切相关，而菌株 BWT-G7 与 （98.9%）的模式菌株形成了一个紧密的聚类。基于 92 个核心基因的基因组分析支持了它们在 16S rRNA 基因系统发育中的关系。基于全基因组相关指数，这两个分离株代表了属内的两个新种。基于这里获得的结果，提出了 sp. nov.（模式菌株 YMB-B2=KCTC 49593=CCM 9151）和 sp. nov.（模式菌株 BWT-G7=KACC 22262=NBRC 115127）。
