Department of Anesthesiology and Reanimation, Erzincan Binali Yildirim University, Mengucek Gazi Training and Research Hospital, Basbaglar District, Erzincan, Turkey.
Gen Physiol Biophys. 2023 Mar;42(2):201-208. doi: 10.4149/gpb_2022055.
Toxic doses of paracetamol are also known to be close to therapeutic doses. This study aimed to biochemically investigate the protective effect of ATP against paracetamol-induced oxidative liver injury in rats and to examine the tissues histopathologically. We divided the animals into the paracetamol alone (PCT), ATP + paracetamol (PATP), and healthy control (HG) groups. Liver tissues were examined biochemically and histopathologically. Malondialdehyde level, AST and ALT activity in the PCT group were significantly higher than those in the HG and PATP groups (p < 0.001). The glutathione (tGSH) level, superoxide dismutase (SOD) and catalase (CAT) activity in the PCT group was significantly lower than that in the HG and PATP groups (p < 0.001), while animal SOD activity was significantly different between the PATP and HG groups (p < 0.001). The activity of CAT was almost the same. In the group treated with paracetamol alone, lipid deposition, necrosis, fibrosis, and grade 3 hydropic degeneration were observed. No histopathological damage was observed of the ATP-treated group, except for grade 2 edema. We discovered that ATP reduces the oxidative stress caused by paracetamol ingestion and protects against paracetamol-induced liver injury at the macroscopic and histological levels.
对乙酰氨基酚的中毒剂量也接近治疗剂量。本研究旨在从生化角度探讨 ATP 对大鼠乙酰氨基酚诱导的氧化性肝损伤的保护作用，并对组织进行组织病理学检查。我们将动物分为单独使用对乙酰氨基酚（PCT）、ATP+对乙酰氨基酚（PATP）和健康对照组（HG）三组。对肝组织进行生化和组织病理学检查。PCT 组的丙二醛水平、AST 和 ALT 活性明显高于 HG 和 PATP 组（p<0.001）。PCT 组的谷胱甘肽（tGSH）水平、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性明显低于 HG 和 PATP 组（p<0.001），而 PATP 和 HG 组动物 SOD 活性差异显著（p<0.001）。CAT 活性几乎相同。在单独使用对乙酰氨基酚的组中，观察到脂质沉积、坏死、纤维化和 3 级水肿变性。除 2 级水肿外，未观察到 ATP 处理组有组织病理学损伤。我们发现，ATP 可减轻乙酰氨基酚摄入引起的氧化应激，并在宏观和组织学水平上保护肝脏免受乙酰氨基酚诱导的损伤。
