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基于细菌反应中心通过有机光收集桥共价结合细胞色素的光转化超分子生物杂化构建体。

Supramolecular Biohybrid Construct for Photoconversion Based on a Bacterial Reaction Center Covalently Bound to Cytochrome by an Organic Light Harvesting Bridge.

机构信息

Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro, Via Orabona, 4, 70126 Bari, Italy.

Istituto per i Processi Chimico Fisici, Consiglio Nazionale delle Ricerche (CNR-IPCF), Via Orabona, 4, 70126 Bari, Italy.

出版信息

Bioconjug Chem. 2023 Apr 19;34(4):629-637. doi: 10.1021/acs.bioconjchem.2c00527. Epub 2023 Mar 10.

DOI:10.1021/acs.bioconjchem.2c00527
PMID:36896985
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10120590/
Abstract

A supramolecular construct for solar energy conversion is developed by covalently bridging the reaction center (RC) from the photosynthetic bacterium and cytochrome (Cyt ) proteins with a tailored organic light harvesting antenna (hCy2). The RC-hCy2-Cyt biohybrid mimics the working mechanism of biological assemblies located in the bacterial cell membrane to convert sunlight into metabolic energy. hCy2 collects visible light and transfers energy to the RC, increasing the rate of photocycle between a RC and Cyt that are linked in such a way that enhances proximity without preventing protein mobility. The biohybrid obtained with average 1 RC/10 hCy2/1.5 Cyt molar ratio features an almost doubled photoactivity versus the pristine RC upon illumination at 660 nm, and ∼10 times higher photocurrent versus an equimolar mixture of the unbound proteins. Our results represent an interesting insight into photoenzyme chemical manipulation, opening the way to new eco-sustainable systems for biophotovoltaics.

摘要

通过共价键将光合细菌的反应中心（RC）与细胞色素（Cyt）蛋白与定制的有机光收集天线（hCy2）连接起来，开发了用于太阳能转换的超分子结构。RC-hCy2-Cyt 生物杂种模拟了位于细菌细胞膜中的生物组装的工作机制，将阳光转化为代谢能。hCy2 收集可见光并将能量传递给 RC，增加了以增强接近而不阻止蛋白质迁移的方式连接的 RC 和 Cyt 之间光循环的速率。以平均 1 RC/10 hCy2/1.5 Cyt 摩尔比获得的生物杂种在 660nm 光照下的光活性比原始 RC 几乎增加了一倍，与未结合蛋白质的等摩尔混合物相比，光电流增加了约 10 倍。我们的结果为光酶化学操纵提供了一个有趣的见解，为生物光伏的新生态可持续系统开辟了道路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a2f/10120590/98953127c6d4/bc2c00527_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a2f/10120590/c481bb843712/bc2c00527_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a2f/10120590/be0e8c4b65cd/bc2c00527_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a2f/10120590/8e45e8cc3d2b/bc2c00527_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a2f/10120590/dbd85c8b6567/bc2c00527_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a2f/10120590/98953127c6d4/bc2c00527_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a2f/10120590/c481bb843712/bc2c00527_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a2f/10120590/be0e8c4b65cd/bc2c00527_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a2f/10120590/8e45e8cc3d2b/bc2c00527_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a2f/10120590/dbd85c8b6567/bc2c00527_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a2f/10120590/98953127c6d4/bc2c00527_0005.jpg

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