Mudoi Kalpataru Dutta, Gogoi Barbi, Borah Gitasree, Hussain Marine, Tasfia Tabassum, Borah Krishnakhi, Lekhak Himangshu, Saikia Siddhartha Proteem
Agrotechnology and Rural Development Division (ARDD), CSIR-North East Institute of Science & Technology, Jorhat, Assam, 785006, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India.
J Genet Eng Biotechnol. 2023 Mar 10;21(1):29. doi: 10.1186/s43141-023-00487-9.
Phoebe goalparensis is an endemic forest species of North East India that belongs to Lauraceae family. P. goalparensis is used as timbers yielding plants for commercial importance in the local furniture markets of North East India. A rapid in vitro micropropagation protocol was established by using apical and axillary shoot tips on Murashige and Skoog medium with varied concentrations of plant growth regulators.
In this study, 5.0 mg/l BAP augmented medium was chosen as the best for shoot multiplication of the plant. However, IBA (2.0 mg/l) was the most responsive for root induction. Moreover, 70% of root induction was recorded during rooting experiment and 80-85% survivability was observed during the acclimatization of this species. Clonal fidelity of P. goalparensis was determined with ISSR marker and it was observed that in vitro raised plantlets were polymonomorphic.
Hence, an efficient protocol with high proliferation and rooting was established for P. Goalparensis that could aid in massive propagation in future.
印度东北部特有的森林树种楠木（Phoebe goalparensis）属于樟科。在印度东北部当地的家具市场上，楠木作为具有商业价值的木材生产植物而被利用。通过在添加不同浓度植物生长调节剂的Murashige和Skoog培养基上使用顶芽和腋芽茎尖，建立了一种快速的离体微繁殖方案。
在本研究中，5.0 mg/l 苄氨基嘌呤（BAP）添加培养基被选为该植物芽增殖的最佳培养基。然而，吲哚丁酸（IBA，2.0 mg/l）对生根诱导反应最灵敏。此外，在生根实验中记录到70%的生根率，在该物种的驯化过程中观察到80 - 85%的成活率。用简单序列重复区间（ISSR）标记测定了楠木的克隆保真度，观察到离体培养的植株是多态性的。
因此，为楠木建立了一种高效的、具有高增殖和生根能力的方案，这有助于未来的大规模繁殖。
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