School of Chemistry and Chemical Engineering, Southwest Petroleum University, Chengdu, Sichuan, 610500, People's Republic of China.
Sichuan Jinmei Environmental Protection Co., Ltd, Chengdu, Sichuan, 610096, People's Republic of China.
Environ Sci Pollut Res Int. 2023 Apr;30(19):55557-55568. doi: 10.1007/s11356-023-25980-z. Epub 2023 Mar 10.
The combination technique of pre-ozonation and bioaugmentation is promising for remediating benzo[a]pyrene (BaP)-contaminated soil. However, little is known about the effect of coupling remediation on the soil biotoxicity, soil respiration, enzyme activity, microbial community structure, and microbial in the process of remediation. This study developed two coupling remediation strategies (pre-ozonation coupled with bioaugmentation by addition of polycyclic aromatic hydrocarbons (PAHs) specific degrading bacteria or activated sludge), compared with sole ozonation and sole bioaugmentation, to improve degradation of BaP and recovery of soil microbial activity and community structure. Results showed that the higher removal efficiency of BaP (92.69-93.19%) was found in coupling remediation, compared with sole bioaugmentation (17.71-23.28%). Meanwhile, coupling remediation significantly reduced the soil biological toxicity, promoted the rebound of microbial counts and activity, and recovered the species numbers and microbial community diversity, compared with sole ozonation and sole bioaugmentation. Besides, it was feasible to replace microbial screening with activated sludge, and coupling remediation by addition of activated sludge was more conducive to the recovery of soil microbial communities and diversity. This work provides a strategy of pre-ozonation coupled with bioaugmentation to further degrade BaP in soil by promoting the rebound of microbial counts and activity, as well as the recovery of species numbers and microbial community diversity.
预臭氧化和生物增强联合技术有望修复苯并[a]芘（BaP）污染土壤。然而，对于耦合修复过程中对土壤生物毒性、土壤呼吸、酶活性、微生物群落结构和微生物的影响知之甚少。本研究开发了两种耦合修复策略（通过添加多环芳烃（PAHs）特异性降解菌或活性污泥的预臭氧化耦合生物增强，与单独臭氧化和单独生物增强相比），以提高 BaP 的降解和土壤微生物活性和群落结构的恢复。结果表明，与单独生物增强（17.71-23.28%）相比，耦合修复中 BaP 的去除效率更高（92.69-93.19%）。同时，与单独臭氧化和单独生物增强相比，耦合修复显著降低了土壤生物毒性，促进了微生物数量和活性的反弹，并恢复了物种数量和微生物群落多样性。此外，用活性污泥代替微生物筛选是可行的，并且添加活性污泥的耦合修复更有利于土壤微生物群落和多样性的恢复。这项工作提供了一种预臭氧化与生物增强相结合的策略，通过促进微生物数量和活性的反弹，以及物种数量和微生物群落多样性的恢复，进一步降解土壤中的 BaP。
