Chen Ziwei, Zuo Qingyang, Liu Caihong, Li Lin, Deliz Quiñones Katherine Y, He Qiang
Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, College of Environment and Ecology, Chongqing University, Chongqing 400044, PR China.
Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, College of Environment and Ecology, Chongqing University, Chongqing 400044, PR China.
Bioresour Technol. 2023 May;376:128838. doi: 10.1016/j.biortech.2023.128838. Epub 2023 Mar 9.
The practical application of solid phase denitrification (SPD) was hindered by either poor water quality from natural plant-like materials or high cost of pure synthetic biodegradable polymers. In this study, by combining polycaprolactone (PCL) with new natural materials (peanut shell, sugarcane bagasse), two novel economical solid carbon sources (SCSs) named as PCL/PS and PCL/SB were developed. Pure PCL and PCL/TPS (PCL with thermal plastic starch) were supplied as controls. During the 162-day operation, especially in the shortest HRT (2 h), higher NO-N removal was achieved by PCL/PS (87.60%±0.06%) and PCL/SB (87.93%±0.05%) compared to PCL (83.28%±0.07%) and PCL/TPS (81.83%±0.05%). The predicted abundance of functional enzymes revealed the potential metabolism pathways of major components of SCSs. The natural components entered the glycolytic cycle by enzymatical generation of intermediates, while biopolymers being converted into small molecule products under specific enzyme activities (i.e., carboxylesterase, aldehyde dehydrogenase), together providing electrons and energy for denitrification.
固相反硝化（SPD）的实际应用受到天然植物类材料水质不佳或纯合成可生物降解聚合物成本高昂的阻碍。在本研究中，通过将聚己内酯（PCL）与新型天然材料（花生壳、甘蔗渣）相结合，开发了两种新型经济的固体碳源（SCSs），分别命名为PCL/PS和PCL/SB。提供纯PCL和PCL/TPS（含热塑性淀粉的PCL）作为对照。在162天的运行期间，特别是在最短水力停留时间（HRT，2小时）下，与PCL（83.28%±0.07%）和PCL/TPS（81.83%±0.05%）相比，PCL/PS（87.60%±0.06%）和PCL/SB（87.93%±0.05%）实现了更高的NO-N去除率。预测的功能酶丰度揭示了SCSs主要成分的潜在代谢途径。天然成分通过酶促生成中间体进入糖酵解循环，而生物聚合物在特定酶活性（即羧酸酯酶、醛脱氢酶）作用下转化为小分子产物，共同为反硝化提供电子和能量。
