Noordwijk Kira J, Chen Leyi, Ruspi Bianca D, Schurer Sydney, Papa Brittany, Fasanello Diana C, McDonough Sean P, Palmer Scott E, Porter Ian R, Basran Parminder S, Donnelly Eve, Reesink Heidi L
Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Department of Materials Science and Engineering, College of Engineering, Cornell University, Ithaca, NY 14853, USA.
Animals (Basel). 2023 Feb 24;13(5):827. doi: 10.3390/ani13050827.
Proximal sesamoid bone (PSB) fracture is the leading cause of fatal musculoskeletal injury in Thoroughbred racehorses in Hong Kong and the US. Efforts are underway to investigate diagnostic modalities that could help identify racehorses at increased risk of fracture; however, features associated with PSB fracture risk are still poorly understood. The objectives of this study were to (1) investigate third metacarpal (MC3) and PSB density and mineral content using dual-energy X-ray absorptiometry (DXA), computed tomography (CT), Raman spectroscopy, and ash fraction measurements, and (2) investigate PSB quality and metacarpophalangeal joint (MCPJ) pathology using Raman spectroscopy and CT. Forelimbs were collected from 29 Thoroughbred racehorse cadavers ( = 14 PSB fracture, = 15 control) for DXA and CT imaging, and PSBs were sectioned for Raman spectroscopy and ash fraction measurements. Bone mineral density (BMD) was greater in MC3 condyles and PSBs of horses with more high-speed furlongs. MCPJ pathology, including palmar osteochondral disease (POD), MC3 condylar sclerosis, and MC3 subchondral lysis were greater in horses with more high-speed furlongs. There were no differences in BMD or Raman parameters between fracture and control groups; however, Raman spectroscopy and ash fraction measurements revealed regional differences in PSB BMD and tissue composition. Many parameters, including MC3 and PSB bone mineral density, were strongly correlated with total high-speed furlongs.
近籽骨（PSB）骨折是香港和美国纯种赛马致命性肌肉骨骼损伤的主要原因。目前正在努力研究有助于识别骨折风险增加的赛马的诊断方法；然而，与PSB骨折风险相关的特征仍知之甚少。本研究的目的是：（1）使用双能X线吸收法（DXA）、计算机断层扫描（CT）、拉曼光谱和灰分测量来研究第三掌骨（MC3）和PSB的密度及矿物质含量；（2）使用拉曼光谱和CT来研究PSB质量和掌指关节（MCPJ）病理。从29具纯种赛马尸体的前肢（14例PSB骨折，15例对照）采集样本进行DXA和CT成像，并将PSB切片进行拉曼光谱和灰分测量。高速弗隆数较多的马匹，其MC3髁和PSB的骨矿物质密度（BMD）更高。高速弗隆数较多的马匹，MCPJ病理情况，包括掌侧骨软骨病（POD）、MC3髁硬化和MC3软骨下溶解更为严重。骨折组和对照组之间的BMD或拉曼参数没有差异；然而，拉曼光谱和灰分测量揭示了PSB BMD和组织组成的区域差异。许多参数，包括MC3和PSB骨矿物质密度，与总高速弗隆数密切相关。
