Valenzuela Cristián A, Azúa Marco, Álvarez Claudio A, Schmitt Paulina, Ojeda Nicolás, Mercado Luis
Grupo de Marcadores Inmunológicos, Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Valparaíso 2374631, Chile.
Laboratorio de Fisiología y Genética Marina, Centro de Estudios Avanzados de Zonas Áridas, Coquimbo 1781421, Chile.
Animals (Basel). 2023 Feb 28;13(5):880. doi: 10.3390/ani13050880.
Autophagy is a fundamental cellular process implicated in the health of the cell, acting as a cytoplasmatic quality control machinery by self-eating unfunctional organelles and protein aggregates. In mammals, autophagy can participate in the clearance of intracellular pathogens from the cell, and the activity of the toll-like receptors mediates its activation. However, in fish, the modulation of autophagy by these receptors in the muscle is unknown. This study describes and characterizes autophagic modulation during the immune response of fish muscle cells after a challenge with intracellular pathogen . For this, primary cultures of muscle cells were challenged with and the expressions of immune markers , , , , , and were analyzed through RT-qPCR. The expressions of several genes involved in autophagy ( and ) were also evaluated with RT-qPCR to understand the autophagic modulation during an immune response. In addition, LC3-II protein content was measured via Western blot. The challenge of trout muscle cells with triggered a concomitant immune response to the activation of the autophagic process, suggesting a close relationship between these two processes.
自噬是一种与细胞健康相关的基本细胞过程，作为一种细胞质质量控制机制，通过自我吞噬无功能的细胞器和蛋白质聚集体发挥作用。在哺乳动物中，自噬可参与清除细胞内的病原体，且Toll样受体的活性介导其激活。然而，在鱼类中，这些受体对肌肉中自噬的调节尚不清楚。本研究描述并表征了细胞内病原体攻击后鱼类肌肉细胞免疫反应期间的自噬调节。为此，用[未提及的物质]对肌肉细胞原代培养物进行攻击，并通过RT-qPCR分析免疫标志物[未提及的标志物名称]的表达。还通过RT-qPCR评估了几种参与自噬的基因（[未提及的基因名称]）的表达，以了解免疫反应期间的自噬调节。此外，通过蛋白质免疫印迹法测量LC3-II蛋白含量。用[未提及的物质]攻击鳟鱼肌肉细胞引发了与自噬过程激活相伴的免疫反应，表明这两个过程之间存在密切关系。
