McGilloway Melissa, Manley Shannon, Aho Alyssa, Heeringa Keisha N, Whitacre Lynsey, Lou Yanping, Squires E James, Pearson Wendy
Department of Animal Biosciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
BioZyme Inc., St. Joseph, MO 64504, USA.
Animals (Basel). 2023 Mar 6;13(5):951. doi: 10.3390/ani13050951.
Equine leaky gut syndrome is characterized by gastrointestinal hyperpermeability and may be associated with adverse health effects in horses. The purpose was to evaluate the effects of a prebiotic product (SUPP) on stress-induced gastrointestinal hyperpermeability. Eight horses received a diet containing SUPP (0.02 g/kg BW) or an unsupplemented diet (CO) (n = 4 per group) for 28 days. On Days 0 and 28, horses were intubated with an indigestible marker of gastrointestinal permeability (iohexol). Half the horses from each feeding group underwent 60 min of transport by trailer immediately followed by a moderate-intensity exercise bout of 30 min (EX), and the remaining horses stayed in stalls as controls (SED). Blood was sampled before iohexol, immediately after trailering, and at 0, 1, 2, 4, and 8 h post-exercise. At the end of the feeding period, horses were washed out for 28 days before being assigned to the opposite feeding group, and the study was replicated. Blood was analyzed for iohexol (HPLC), lipopolysaccharide (ELISA), and serum amyloid A (latex agglutination assay). Data were analyzed using three-way and two-way ANOVA. On Day 0, the combined challenge of trailer transport and exercise significantly increased plasma iohexol in both feeding groups; this increase was not seen in SED horses. On Day 28, EX increased plasma iohexol only in the CO feeding group; this increase was completely prevented by the provision of SUPP. It is concluded that combined transport and exercise induce gastrointestinal hyperpermeability. Dietary SUPP prevents this and therefore may be a useful prophylactic for pathologies associated with gastrointestinal hyperpermeability in horses.
马的肠漏综合征的特征是胃肠道通透性增加，可能对马的健康产生不良影响。本研究旨在评估一种益生元产品（SUPP）对应激诱导的胃肠道通透性增加的影响。八匹马接受了含SUPP（0.02 g/kg体重）的日粮或未添加SUPP的日粮（CO）（每组n = 4），持续28天。在第0天和第28天，给马灌胃一种不可消化的胃肠道通透性标志物（碘海醇）。每个饲喂组的一半马立即通过拖车运输60分钟，随后进行30分钟的中等强度运动（EX），其余马留在厩中作为对照（SED）。在灌胃碘海醇前、拖车运输后立即以及运动后0、1、2、4和8小时采集血液样本。在饲喂期结束时，让马洗脱28天，然后分配到相反的饲喂组，重复该研究。分析血液中的碘海醇（高效液相色谱法）、脂多糖（酶联免疫吸附测定法）和血清淀粉样蛋白A（乳胶凝集试验）。数据采用三因素和两因素方差分析进行分析。在第0天，拖车运输和运动的联合刺激显著增加了两个饲喂组的血浆碘海醇水平；在SED组的马中未观察到这种增加。在第28天，EX仅在CO饲喂组中增加了血浆碘海醇水平；提供SUPP完全阻止了这种增加。得出的结论是，运输和运动联合诱导胃肠道通透性增加。日粮中的SUPP可预防这种情况，因此可能是预防马胃肠道通透性增加相关病症的有用药物。
