Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, School of Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510006, China.
School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Int J Mol Sci. 2023 Mar 1;24(5):4711. doi: 10.3390/ijms24054711.
Lead-free perovskite is one of the ideal solutions for the toxicity and instability of lead halide perovskite quantum dots. As the most ideal lead-free perovskite at present, bismuth-based perovskite quantum dots still have the problem of a low photoluminescence quantum yield, and its biocompatibility also needs to be explored. In this paper, Ce ions were successfully introduced into the CsBiCl lattice using a modified antisolvent method. The photoluminescence quantum yield of CsBiCl:Ce is up to 22.12%, which is 71% higher than that of undoped CsBiCl. The two quantum dots show high water-soluble stability and good biocompatibility. Under the excitation of a 750 nm femtosecond laser, high-intensity up-conversion fluorescence images of human liver hepatocellular carcinoma cells cultured with the quantum dots were obtained, and the fluorescence of the two quantum dots was observed in the image of the nucleus. The fluorescence intensity of cells cultured with CsBiCl:Ce was 3.20 times of that of the control group and 4.54 times of the control group for the fluorescence intensity of the nucleus, respectively. This paper provides a new strategy to develop the biocompatibility and water stability of perovskite and expands the application of perovskite in the field.
无铅钙钛矿是解决卤化铅钙钛矿量子点毒性和不稳定性的理想方案之一。目前，最理想的无铅钙钛矿是铋基钙钛矿量子点，但仍存在荧光量子产率低的问题，其生物相容性也需要进一步探索。本文采用改进的抗溶剂法成功地将 Ce 离子引入到 CsBiCl 晶格中。CsBiCl:Ce 的荧光量子产率高达 22.12%，比未掺杂的 CsBiCl 高 71%。这两种量子点具有高水溶性稳定性和良好的生物相容性。在 750nm 飞秒激光激发下，获得了用量子点培养的人肝癌细胞的高强度上转换荧光图像，并观察到了两种量子点在细胞核图像中的荧光。用 CsBiCl:Ce 培养的细胞的荧光强度分别是对照组的 3.20 倍和细胞核荧光强度的 4.54 倍。本文为开发钙钛矿的生物相容性和水分稳定性提供了新策略，并扩展了钙钛矿在该领域的应用。
