金属响应转录因子共同调控抗西格玛因子（Rsd）和核糖体二聚体因子的表达。
Metal-Responsive Transcription Factors Co-Regulate Anti-Sigma Factor (Rsd) and Ribosome Dimerization Factor Expression.
机构信息
Department of Physics, Osaka Medical and Pharmaceutical University, Takatsuki 569-8686, Osaka, Japan.
School of Agriculture, Meiji University, Kawasaki 214-8571, Kanagawa, Japan.
出版信息
Int J Mol Sci. 2023 Mar 1;24(5):4717. doi: 10.3390/ijms24054717.
Bacteria exposed to stress survive by regulating the expression of several genes at the transcriptional and translational levels. For instance, in , when growth is arrested in response to stress, such as nutrient starvation, the anti-sigma factor Rsd is expressed to inactivate the global regulator RpoD and activate the sigma factor RpoS. However, ribosome modulation factor (RMF) expressed in response to growth arrest binds to 70S ribosomes to form inactive 100S ribosomes and inhibit translational activity. Moreover, stress due to fluctuations in the concentration of metal ions essential for various intracellular pathways is regulated by a homeostatic mechanism involving metal-responsive transcription factors (TFs). Therefore, in this study, we examined the binding of a few metal-responsive TFs to the promoter regions of and through promoter-specific TF screening and studied the effects of these TFs on the expression of and in each TF gene-deficient strain through quantitative PCR, Western blot imaging, and 100S ribosome formation analysis. Our results suggest that several metal-responsive TFs (CueR, Fur, KdpE, MntR, NhaR, PhoP, ZntR, and ZraR) and metal ions (Cu, Fe, K, Mn, Na, Mg, and Zn) influence and gene expression while regulating transcriptional and translational activities.
细菌通过在转录和翻译水平上调节几个基因的表达来应对压力。例如，在 中，当生长因应激而停滞时，例如营养饥饿，反西格玛因子 Rsd 被表达以失活全局调节因子 RpoD 并激活 sigma 因子 RpoS。然而，响应生长停滞而表达的核糖体调节因子 (RMF) 与 70S 核糖体结合形成无活性的 100S 核糖体并抑制翻译活性。此外，由于对各种细胞内途径至关重要的金属离子浓度的波动而产生的应激通过涉及金属反应转录因子 (TF) 的动态平衡机制进行调节。因此，在这项研究中，我们通过启动子特异性 TF 筛选检查了几种金属反应 TF 与 和 启动子区域的结合，并通过定量 PCR、Western blot 成像和 100S 核糖体形成分析研究了这些 TF 在每种 TF 基因缺陷 菌株中对 和 表达的影响。我们的结果表明，几种金属反应 TF（CueR、Fur、KdpE、MntR、NhaR、PhoP、ZntR 和 ZraR）和金属离子（Cu、Fe、K、Mn、Na、Mg 和 Zn）影响 和 基因表达，同时调节转录和翻译活性。
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