Department of Genetic, Microbiology and Statistic, University of Barcelona, Diagonal 643, 08028 Barcelona, Spain.
Laboratory of Microbial Immunochemistry and Vaccines, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland.
Int J Mol Sci. 2023 Mar 1;24(5):4768. doi: 10.3390/ijms24054768.
The genus presents five different pathogenic species: , , , and . These species cause infections mainly in fish, but they can also infect reptiles, birds or humans. Lipopolysaccharide (endotoxin) plays an important role in the pathogenesis of these bacteria. For the first time, the chemical structure and genomics of the lipopolysaccharide (LPS) core oligosaccharides of , , and were studied. The complete gene assignments for all core biosynthesis gene functions were acquired. The structure of core oligosaccharides was investigated by ¹H and C nuclear magnetic resonance (NMR) spectroscopy. The structures of and core oligosaccharides show the presence of →3,4)-L--α-D--Hep, two terminal β-D-Glc, →2,3,7)-L--α-D--Hep, →7)-L--α-D--Hep, terminal α-D-GlcN, two →4)-α-D-GalA, → 3)-α-D-GlcNAc, terminal β-D-Gal and →5-substituted Kdo. core oligosaccharide shows only one terminal β-D-Glc, and instead of terminal β-D-Gal a terminal α-D-GlcNAc. core oligosaccharide shows only one terminal β-D-Glc, one →4)-α-D-GalA and do not have terminal α-D-GlcN (see complementary figure).
， ， ， 和 。这些物种主要感染鱼类，但也可以感染爬行动物、鸟类或人类。脂多糖（内毒素）在这些细菌的发病机制中起着重要作用。首次研究了 ， ， 和 的脂多糖（LPS）核心寡糖的化学结构和基因组学。获得了所有核心生物合成基因功能的完整基因分配。通过 ¹H 和 C 核磁共振（NMR）光谱研究了核心寡糖的结构。和 的核心寡糖结构显示存在→3,4)-L--α-D--Hep、两个末端β-D-Glc、→2,3,7)-L--α-D--Hep、→7)-L--α-D--Hep、末端α-D-GlcN、两个→4)-α-D-GalA、→ 3)-α-D-GlcNAc、末端β-D-Gal 和→5-取代的 Kdo。 核心寡糖仅显示一个末端β-D-Glc，而不是末端β-D-Gal 而是末端α-D-GlcNAc。 核心寡糖仅显示一个末端β-D-Glc、一个→4)-α-D-GalA，并且没有末端α-D-GlcN（见补充图）。
Zotero 插件
