Müller-Loennies S, Holst O, Brade H
Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, Germany.
Eur J Biochem. 1994 Sep 1;224(2):751-60. doi: 10.1111/j.1432-1033.1994.t01-1-00751.x.
The lipopolysaccharide of Escherichia coli J-5 was sequentially de-O-acylated, dephosphorylated, reduced, de-N-acylated, and N-acetylated. The products were separated by high-performance anion-exchange chromatography into a nonasaccharide (1), two octasaccharides (2, 3), and a heptasaccharide (4). Compositional analysis, methylation analysis, and NMR spectroscopy revealed the structures of the products as: alpha-D-GlcpNAc-(1-7)-L-alpha-D-Hepp-(1-7)-[alpha-D-Glcp-(1-3)-]-L -alpha-D- Hepp-(1-3)-R1, (1) L-alpha-D-Hepp-(1-7)-[alpha-D-Glcp-(1-3)-]-L-alpha-D-Hepp-(1 -3)-R1, (2) alpha-D-GlcpNAc-(1-7)-L-alpha-D-Hepp-(1-7)-[alpha-D-Glcp-(1-3)-]-L -alpha-D- Hepp-(1-3)-R2, (3) alpha-D-Glcp-(1-3)-L-alpha-D-Hepp-(1-3)-R1, (4) in which 1R is L-alpha-D-Hepp-(1-5)-[alpha-Kdop-(2-4)-]-alpha-Kdop-(2 -6)- beta-D-GlcpNAc-(1-6)-D-GlcN-Acol, and 2R is L-alpha-D-Hepp-(1-5)-alpha-Kdop-(2-6)-beta-D-GlcpNAc-(1-6 )-D- GlcNAcol (LD-Hep, L-glycero-D-manno-heptose; Kdo, 3-deoxy-D-manno-octulopyranosonic acid; GlcNAcol, 2-acetamido-2-deoxy-glucitol). Fast-atom-bombardment mass spectrometry of de-O-acylated and dephosphorylated lipopolysaccharide showed that the isolated oligosaccharides represented the complete carbohydrate moiety of the lipopolysaccharide, and indicated that the non-reducing terminal D-GlcN residue in lipopolysaccharide was present as the free base.
大肠杆菌J-5的脂多糖依次进行了脱O-酰化、脱磷酸化、还原、脱N-酰化和N-乙酰化反应。产物通过高效阴离子交换色谱分离为一种九糖（1）、两种八糖（2、3）和一种七糖（4）。组成分析、甲基化分析和核磁共振光谱确定了产物的结构如下：α-D-葡萄糖胺-（1-7）-L-α-D-庚糖-（1-7）-[α-D-葡萄糖-（1-3）-]-L-α-D-庚糖-（1-3）-R1，（1）L-α-D-庚糖-（1-7）-[α-D-葡萄糖-（1-3）-]-L-α-D-庚糖-（1-3）-R1，（2）α-D-葡萄糖胺-（1-7）-L-α-D-庚糖-（1-7）-[α-D-葡萄糖-（1-3）-]-L-α-D-庚糖-（1-3）-R2，（3）α-D-葡萄糖-（1-3）-L-α-D-庚糖-（1-3）-R1，（4）其中1R为L-α-D-庚糖-（1-5）-[α-Kdo-（2-4）-]-α-Kdo-（2-6）-β-D-葡萄糖胺-（1-6）-D-葡糖胺醇，2R为L-α-D-庚糖-（1-5）-α-Kdo-（2-6）-β-D-葡萄糖胺-（1-6）-D-葡糖胺醇（LD-Hep，L-甘油-D-甘露庚糖；Kdo，3-脱氧-D-甘露辛吡喃糖酸；GlcNAcol，2-乙酰氨基-2-脱氧葡糖醇）。脱O-酰化和脱磷酸化脂多糖的快原子轰击质谱表明，分离得到的寡糖代表了脂多糖完整的碳水化合物部分，并表明脂多糖中非还原末端的D-葡糖胺残基以游离碱形式存在。
