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转录组学揭示 对磷胁迫响应的机制

Transcriptomics Insights into Phosphorus Stress Response of .

机构信息

Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.

College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China.

出版信息

Int J Mol Sci. 2023 Mar 2;24(5):4874. doi: 10.3390/ijms24054874.

Abstract

Through excellent absorption and transformation, the macrophyte () can considerably remove phosphorus from wastewater. The results of changes in growth rate, chlorophyll content, and roots number and length showed that could cope better with high phosphorus stress compared with low phosphorus stress. Transcriptome and differentially expressed genes (DEGs) analyses revealed that, when exposed to phosphorus stresses at various concentrations, the roots were more active than the leaves, with more DEGs regulated. also showed different gene expression and pathway regulatory patterns when exposed to low phosphorus and high phosphorus stresses. 's capacity to cope with phosphorus stress was maybe due to its improved ability to regulate metabolic pathways such as photosynthesis, oxidative stress reduction, phosphorus metabolism, signal transduction, secondary metabolites biosynthesis, and energy metabolism. In general, has a complex and interconnected regulatory network that deals efficiently with phosphorus stress to varying degrees. This is the first time that the mechanisms of in sustaining phosphorus stress have been fully examined at the transcriptome level using high-throughput sequencing analysis, which may indicate the direction of follow-up research and have some guiding value for its future applications.

摘要

通过优异的吸收和转化能力,大型水生植物()可以从废水中大量去除磷。生长速率、叶绿素含量、根系数量和长度变化的结果表明,与低磷胁迫相比,能更好地应对高磷胁迫。转录组和差异表达基因(DEGs)分析表明,当暴露于不同浓度的磷胁迫时,根比叶更活跃,受调控的 DEGs 更多。当暴露于低磷和高磷胁迫时,也表现出不同的基因表达和途径调控模式。可能是因为其调节光合作用、氧化应激减轻、磷代谢、信号转导、次生代谢物生物合成和能量代谢等代谢途径的能力得到了提高,从而增强了应对磷胁迫的能力。总的来说,具有复杂而相互关联的调控网络,能有效地应对不同程度的磷胁迫。这是首次使用高通量测序分析在转录组水平上全面研究应对磷胁迫的机制,这可能为后续研究指明方向,并对其未来应用具有一定的指导价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c75f/10003231/b476660f7ae0/ijms-24-04874-g001.jpg

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