Gibson M J, Moscovitz H C, Kokoris G J, Silverman A J
Department of Medicine, Mount Sinai School of Medicine, New York, NY 10029.
Brain Res. 1987 Oct 20;424(1):133-8. doi: 10.1016/0006-8993(87)91202-9.
Hypogonadal female mice, genetically deficient in gonadotropin releasing hormone (GnRH), respond to preoptic area (POA) grafts obtained from normal fetal or neonatal mice with increased gonadotropin levels, ovarian and uterine development and continual vaginal estrus rather than spontaneous ovulatory cyclicity. Previous studies showed that such mice became pregnant following one overnight pairing with a normal male, indicating reflex ovulation. The present study evaluated plasma LH concentrations in relation to mating. Plasma LH levels in the hpg females with POA grafts were significantly elevated 10 min following the male partner's ejaculation, but were no different than baseline at 30, 60 or 120 min following the male's ejaculation. The post-copulatory plasma LH levels of 3.0 +/- 0.6 ng/ml (mean +/- S.E.M.) were considerably lower than the proestrous LH surge seen in the normal females in the colony (16.8 +/- 4.8 ng/ml), but in at least 4 of 10 hpg mice the levels were sufficient to induce ovulation as proved by pregnancy following this single mating. Grafts contained GnRH-reactive cells and fibers that projected to the median eminence of the host brains.
性腺功能减退的雌性小鼠，因基因缺陷缺乏促性腺激素释放激素（GnRH），对取自正常胎儿或新生小鼠的视前区（POA）移植有反应，表现为促性腺激素水平升高、卵巢和子宫发育以及持续的阴道发情，而非自发排卵周期。先前的研究表明，此类小鼠与正常雄性小鼠过夜交配一次后即可怀孕，表明为反射性排卵。本研究评估了与交配相关的血浆促黄体生成素（LH）浓度。有POA移植的hpg雌性小鼠在雄性伴侣射精后10分钟时血浆LH水平显著升高，但在雄性射精后30、60或120分钟时与基线水平无差异。交配后血浆LH水平为3.0 +/- 0.6 ng/ml（平均值 +/- 标准误），远低于群体中正常雌性小鼠发情前期的LH峰（16.8 +/- 4.8 ng/ml），但在10只hpg小鼠中至少有4只的水平足以诱导排卵，单次交配后怀孕即证明了这一点。移植组织含有GnRH反应性细胞和纤维，这些细胞和纤维投射到宿主脑的正中隆起。
