Sharma R, Timiras P S
Department of Physiology-Anatomy, University of California, Berkeley 94720.
Brain Res. 1987 Dec 1;433(2):285-7. doi: 10.1016/0165-3806(87)90033-2.
The binding of [3H]dexamethasone-receptor complexes to purified nuclei was studied in the cerebral hemispheres of immature (3-week-old) and mature (26-week-old) Long-Evans male rats to determine the age-related changes, if any, in the physicochemical properties of glucocorticoid receptors. Our data show that heat activation (for 45 min at 25 degrees C) significantly enhances the nuclear binding of [3H]dexamethasone-receptor complexes in rats of both ages, with a greater magnitude in immature rats. Ca2+ activation (20 mM Ca2+ for 45 min at 0 degree C) also enhances the nuclear binding of bound receptor complexes but to a similar extent at both ages. These findings indicate that some of the physicochemical properties (e.g. heat activation) of glucocorticoid receptor change, while others (e.g. Ca2+ activation) remain unchanged at different phases of the lifespan.
在未成熟（3周龄）和成熟（26周龄）的Long-Evans雄性大鼠的大脑半球中，研究了[3H]地塞米松-受体复合物与纯化细胞核的结合情况，以确定糖皮质激素受体的物理化学性质是否存在与年龄相关的变化。我们的数据表明，热激活（在25℃下45分钟）显著增强了两个年龄段大鼠中[3H]地塞米松-受体复合物的核结合，未成熟大鼠中的增强幅度更大。Ca2+激活（在0℃下20 mM Ca2+处理45分钟）也增强了结合受体复合物的核结合，但在两个年龄段的增强程度相似。这些发现表明，糖皮质激素受体的一些物理化学性质（如热激活）会发生变化，而其他性质（如Ca2+激活）在生命周期的不同阶段保持不变。
