Lin Yongsheng, Qiu Ziwen, Lin Xiaojie, Wu Yingxiang, Niu Xianqian, Yin Guanwen, Shao Dandan, Xiang Xuwen, Li Yongyu, Yang Chao
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Institute of Natural Products of Horticultural Plants, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Plants (Basel). 2023 Feb 24;12(5):1026. doi: 10.3390/plants12051026.
Many aromatic plant volatile compounds contain methyleugenol, which is an attractant for insect pollination and has antibacterial, antioxidant, and other properties. The essential oil of leaves contains 90.46% methyleugenol, which is an ideal material for studying the biosynthetic pathway of methyleugenol. Eugenol synthase (EGS) is one of the key enzymes involved in the synthesis of methyleugenol. We recently reported two eugenol synthase genes ( and ) present in , where and were mainly expressed in flowers, followed by leaves, and had the lowest expression levels in stems. In this study, the functions of and in the biosynthesis of methyleugenol were investigated using transient gene expression technology and virus-induced gene silencing (VIGS) technology in . Here, in the genes overexpression group, the transcription levels of the gene and gene were increased 13.46 times and 12.47 times, respectively, while the methyleugenol levels increased 18.68% and 16.48%. We further verified the function of the genes by using VIGS, as the transcript levels of the and genes were downregulated by 79.48% and 90.35%, respectively, and the methyleugenol content in decreased by 28.04% and 19.45%, respectively. The results indicated that the and genes were involved in the biosynthesis of methyleugenol, and the transcript levels of the and genes correlated with the methyleugenol content in .
许多芳香植物挥发性化合物含有甲基丁香酚,它是昆虫授粉的引诱剂,具有抗菌、抗氧化等特性。叶片精油中甲基丁香酚含量为90.46%,是研究甲基丁香酚生物合成途径的理想材料。丁香酚合酶(EGS)是参与甲基丁香酚合成的关键酶之一。我们最近报道了存在于[植物名称未给出]中的两个丁香酚合酶基因([基因名称未给出]和[基因名称未给出]),其中[基因名称未给出]和[基因名称未给出]主要在花中表达,其次是在叶中表达,在茎中的表达水平最低。在本研究中,利用瞬时基因表达技术和病毒诱导基因沉默(VIGS)技术在[植物名称未给出]中研究了[基因名称未给出]和[基因名称未给出]在甲基丁香酚生物合成中的功能。在此,在[基因名称未给出]基因过表达组中,[基因名称未给出]基因和[基因名称未给出]基因的转录水平分别提高了13.46倍和12.47倍,而甲基丁香酚水平分别提高了18.68%和16.48%。我们通过VIGS进一步验证了[基因名称未给出]基因的功能,因为[基因名称未给出]和[基因名称未给出]基因的转录水平分别下调了79.48%和90.35%,[植物名称未给出]中的甲基丁香酚含量分别下降了28.04%和19.45%。结果表明,[基因名称未给出]和[基因名称未给出]基因参与了甲基丁香酚的生物合成,且[基因名称未给出]和[基因名称未给出]基因的转录水平与[植物名称未给出]中的甲基丁香酚含量相关。