Department of Animal Physiology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Manastur No. 3-5, 400372 Cluj-Napoca, Romania.
Department of Histology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Manastur No. 3-5, 400372 Cluj-Napoca, Romania.
Nutrients. 2023 Feb 23;15(5):1105. doi: 10.3390/nu15051105.
Gastrointestinal microbiota can be easily altered by common treatments, such as antibiotic therapy. However, the dysmicrobism induced by such a treatment may be counteracted by the administration of different beneficial microbes, such as probiotics. Therefore, this study aimed to establish the interaction between intestinal microbiota, antibiotic therapy, and sporulated bacteria, correlated with the evolution of growth indices. Twenty-five Wistar rats, females, were divided into five groups. Amoxicillin and a probiotic combination of , and were administered according to each group's purpose. The conventional growth indices were calculated and histological and immunohistochemical assessments were realized from intestinal samples. The results of the conventional growth indices suggested a beneficial effect when the antibiotic therapy was accompanied by probiotics, while for the groups where the dysmicrobism was present, the values for feed conversion ratio were negative. These findings were supported by the microscopic aspects of the intestinal mucosa, which suggested a decreased absorption capacity due to significant morphological changes. Moreover, the immunohistochemical reaction of the inflammatory cells from intestinal lamina propria was intensely positive for the same affected groups. However, for the control group and the group with antibiotic and probiotic treatment, the immunopositivity was significantly decreased. Probiotics based on spores administered simultaneously with the antibiotic offered the best restoration of the gut microbiota, a fact suggested by the absence of intestinal lesions, a normal food conversion ratio, and low expression of TLR4 and LBP immunomarkers.
肠道微生物群很容易被常见的治疗方法改变，如抗生素治疗。然而，这种治疗引起的微生物失调可以通过给予不同的有益微生物来抵消，如益生菌。因此，本研究旨在建立肠道微生物群、抗生素治疗和芽孢杆菌之间的相互作用，与生长指数的演变相关。将 25 只雌性 Wistar 大鼠分为五组。根据每组的目的，给予阿莫西林和 、 和 的益生菌组合。计算常规生长指数，并从肠道样本中进行组织学和免疫组织化学评估。常规生长指数的结果表明，抗生素治疗时伴随使用益生菌有有益的效果，而对于存在微生物失调的组，饲料转化率值为负。这些发现得到了肠黏膜微观方面的支持，这表明由于形态发生了显著变化，吸收能力下降。此外，肠固有层炎症细胞的免疫组织化学反应对受影响的相同组呈强烈阳性。然而，对于对照组和抗生素和益生菌治疗组，免疫阳性率显著降低。同时给予抗生素的基于 芽孢的益生菌提供了最佳的肠道微生物群恢复，这一事实表明肠道没有病变，食物转化率正常，TLR4 和 LBP 免疫标志物的表达较低。
