Charles University, Faculty of Science, Department of Analytical Chemistry, Albertov 6, 12800 Prague, Czech Republic.
FZU-Institute of Physics of the Czech Academy of Sciences, Na Slovance 1999/2, 18221 Prague, Czech Republic.
Sensors (Basel). 2023 Mar 3;23(5):2776. doi: 10.3390/s23052776.
A novel electrochemical approach using two different electrode materials, platinum and boron-doped diamond (BDD), was employed to study the oxidative stability of the drug abacavir. Abacavir samples were subjected to oxidation and subsequently analysed using chromatography with mass detection. The type and amount of degradation products were evaluated, and results were compared with traditional chemical oxidation using 3% hydrogen peroxide. The effect of pH on the rate of degradation and the formation of degradation products were also investigated. In general, both approaches led to the same two degradation products, identified using mass spectrometry, and characterised by 319.20 and m/z 247.19. Similar results were obtained on a large-surface platinum electrode at a potential of +1.15 V and a BDD disc electrode at +4.0 V. Degradation of 20% of abacavir, the rate required for pharmaceutical stability studies, took only a few minutes compared to hours required for oxidation with hydrogen peroxide. Measurements further showed that electrochemical oxidation in ammonium acetate on both types of electrodes is strongly pHdependent. The fastest oxidation was achieved at pH 9. The pH also affects the composition of the products, which are formed in different proportions depending on the pH of the electrolyte.
采用两种不同的电极材料（铂和掺硼金刚石（BDD））的新型电化学方法用于研究药物阿巴卡韦的氧化稳定性。将阿巴卡韦样品进行氧化，然后使用带有质量检测的色谱法进行分析。评估了降解产物的类型和数量，并将结果与使用 3%过氧化氢的传统化学氧化进行了比较。还研究了 pH 值对降解速率和降解产物形成的影响。一般来说，这两种方法都导致了相同的两种降解产物，通过质谱鉴定，并通过 319.20 和 m/z 247.19 进行了特征化。在 +1.15 V 的大表面积铂电极和 +4.0 V 的 BDD 圆盘电极上也获得了相似的结果。与使用过氧化氢氧化所需的几个小时相比，仅需几分钟即可使阿巴卡韦降解 20%，这是药物稳定性研究所需的降解率。测量结果进一步表明，在两种类型的电极上，在乙酸铵中的电化学氧化强烈依赖于 pH 值。在 pH 值为 9 时，氧化速度最快。pH 值还会影响产物的组成，产物的形成比例取决于电解质的 pH 值。
