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冻存管冷冻法和 OPS 玻璃化法冻存小鼠原始卵泡的复苏后活力。

Post-thaw viability of mouse preantral follicles after cryopreservation with cryotube freezing and OPS vitrification procedures.

机构信息

University of Veterinary Medicine Budapest Department of Obstetrics and Food Animal Medicine Clinic, István st. 2, 1078 Budapest, Hungary.

University of Veterinary Medicine Budapest Department of Obstetrics and Food Animal Medicine Clinic, István st. 2, 1078 Budapest, Hungary.

出版信息

Reprod Biol. 2023 Jun;23(2):100752. doi: 10.1016/j.repbio.2023.100752. Epub 2023 Mar 9.

DOI:10.1016/j.repbio.2023.100752
PMID:36905824
Abstract

In the field of reproductive science, there is an increased interest in the application of ovarian preantral follicles. Since the ovary contains a great amount of preantral follicles (PAF), the cryopreservation and in vitro culture of such follicles support the fertility preservation of domestic animals with high genetic value, endangered or zoo animals, and women before anticancer therapy. To date, no standard freezing or vitrification protocol is available in human or animals. The aim of the present study was to examine the viability of preantral follicles cryopreserved using freezing or vitrification protocols: cryotube freezing or OPS vitrification.

摘要

在生殖科学领域,人们对应用卵巢小腔前卵泡越来越感兴趣。由于卵巢中含有大量的小腔前卵泡(PAF),因此冷冻保存和体外培养这些卵泡可以支持具有高遗传价值的家畜、濒危或动物园动物以及接受抗癌治疗前的女性的生育力保存。迄今为止,在人类或动物中尚无标准的冷冻或玻璃化方案。本研究的目的是检查使用冷冻或玻璃化方案(Cryotube 冷冻或 OPS 玻璃化)冷冻保存的小腔前卵泡的活力。

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