School of Basic Medical Sciences, Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou, 510006, PR China; School of Life Sciences and Biopharmaceutics, Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou, 510006, PR China.
School of Life Sciences and Biopharmaceutics, Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou, 510006, PR China.
Chemosphere. 2023 May;324:138377. doi: 10.1016/j.chemosphere.2023.138377. Epub 2023 Mar 9.
Phytoremediation is a widely accepted bioremediation method of treating heavy metal contaminated soils. Nevertheless, the remediation efficiency in multi-metal contaminated soils is still unsatisfactory attributable to susceptibility to different metals. To isolate root-associated fungi for improving phytoremediation efficiency in multi-metal contaminated soils, the fungal flora in root endosphere, rhizoplane, rhizosphere of Ricinus communis L. in heavy metal contaminated soils and non-heavy metal contaminated soils were compared by ITS amplicon sequencing, and then the critical fungal strains were isolated and inoculated into host plants to improve phytoremediation efficiency in Cd, Pb, and Zn-contaminated soils. The fungal ITS amplicon sequencing analysis indicated that the fungal community in root endosphere was more susceptible to heavy metals than those in rhizoplane and rhizosphere soils and Fusarium dominated the endophytic fungal community of R. communis L. roots under heavy metal stress. Three endophytic strains (Fusarium sp. F2, Fusarium sp. F8, and Fusarium sp. F14) isolated from Ricinus communis L. roots showed high resistances to multi-metals and possessed growth-promoting characteristics. Biomass and metal extraction amount of R. communis L. with Fusarium sp. F2, Fusarium sp. F8, and Fusarium sp. F14 inoculation in Cd-, Pb- and Zn-contaminated soils were significantly higher than those without the inoculation. The results suggested that fungal community analysis-guided isolation could be employed to obtain desired root-associated fungi for enhancing phytoremediation of multi-metal contaminated soils.
植物修复是一种广泛接受的生物修复方法，用于处理重金属污染土壤。然而，在多金属污染土壤中的修复效率仍然不尽如人意，这归因于对不同金属的敏感性。为了分离根相关真菌，以提高多金属污染土壤的植物修复效率，通过 ITS 扩增子测序比较了重金属污染土壤和非重金属污染土壤中蓖麻根内、根际和根际的真菌菌群，然后分离关键真菌菌株并接种到宿主植物中，以提高 Cd、Pb 和 Zn 污染土壤的植物修复效率。真菌 ITS 扩增子测序分析表明，根内真菌群落比根际和根际土壤更易受重金属影响，并且在重金属胁迫下，镰孢菌(Fusarium)主导了蓖麻根的内生真菌群落。从蓖麻根中分离出的三种内生菌株（Fusarium sp. F2、Fusarium sp. F8 和 Fusarium sp. F14）对多种金属具有高抗性，并具有促进生长的特性。接种 Fusarium sp. F2、Fusarium sp. F8 和 Fusarium sp. F14 的蓖麻生物量和金属提取量在 Cd、Pb 和 Zn 污染土壤中均显著高于未接种的。结果表明，基于真菌群落分析的分离可以获得所需的根相关真菌，以增强多金属污染土壤的植物修复。
